Effects of Shenggu Zaizao Pills on related indexes of bone morphometry in steroid-induced necrosis of the femoral head in rabbits

doi:10.13418/j.issn.1001-165x.2022.5.10

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (5) : 555-561. DOI: 10.13418/j.issn.1001-165x.2022.5.10

Effects of Shenggu Zaizao Pills on related indexes of bone morphometry in steroid-induced necrosis of the femoral head in rabbits

Ma Xueqiang¹, Cao Linzhong ^1,2*, Jiang Wei ², Zhang Qi ², Liu Mengchu ¹, Wang Duoxian ¹

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Abstract

Objective To observe the intervention effect of Shenggu Zaizao Pills on steroid-induced necrosis of the femoral head (SONFH) rabbit femoral head histomorphology and related indexes of osteometry. Methods Twenty female New Zealand rabbits were randomly divided into a normal group, a model group, a Shenggu Zaizao pill group and a positive control drug Xianling Gubao capsule group, 5 in each group. Except the normal group, the other three groups received endotoxin combined with methylprednisone sodium succinate injection to modeling. After 28d, Shenggu Zaizao Pills group was given 2.4 g/kg Shenggu Zaizai Pills, Xianling Gubao Capsule group was given 0.4 g/kg Xianling Gubao Capsule, normal group and model group were given 10mL of normal saline, 1 time/d. After 28d, the bilateral femoral heads of the rabbits in each group were taken to perform Micro-CT scanning to analyze osteometric parameters. HE staining was used to observe the pathological changes of the femoral head tissue morphology. Results Compared with the normal group, Micro-CT scan analysis showed that the femoral head bone mineral density (BMD),bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th) and connectivity density (Conn.D) in the model group significantly decreased, while trabecular bone separation (Tb.Sp) and structural model index (SMI) significantly increased (P<0.05). Microscopic observation of HE stained pathological sections showed that the rate of empty bone lacunae and the area of adipocytes in the model group significantly increased, the epiphysis was deformed, and the trabecular bone was hyperplasia, narrowed or broken. Compared with the model group, the osteometric and histopathological changes of the femoral head of the rabbits in the Shenggu Zaizao Pills group and Xianling Gubao Capsule group significantly improved (P<0.05). There was no significant difference in curative effect between Shenggu Zaizao Pills group and Xianling Gubao Capsules group (P>0.05). Conclusions Shenggu Zaizao Pills can significantly increase the level of SONFH osteometric indexes in rabbits and improve histopathological morphology, thereby achieving the effect of treating SONFH.

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Steroid-induced femoral head necrosis; / / Shenggu Zaizao Pills; / / Bone tissue morphology / Bone measurement; / / Rabbit modeling

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Ma Xueqiang, Cao Linzhong, Jiang Wei, Zhang Qi , Liu Mengchu, Wang Duoxian. Effects of Shenggu Zaizao Pills on related indexes of bone morphometry in steroid-induced necrosis of the femoral head in rabbits[J]. Chinese Journal of Clinical Anatomy. 2022, 40(5): 555-561 https://doi.org/10.13418/j.issn.1001-165x.2022.5.10

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