Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p&nbsp;

doi:10.13418/j.issn.1001-165x.2022.3.08

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (3) : 286-291. DOI: 10.13418/j.issn.1001-165x.2022.3.08

Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p

Shi Haoran^1#, Bao Guanglong^1#, LingHu Xitao¹, Wu Honghan¹, Zhao Zeju², Liu Xiao³, Fu Yao⁴ , Huang Shuai⁵*, Wa Qingde¹*

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Abstract

【Abstract】 Objective To explore the effect and mechanism of oridonin on the migration and invasion of PC-3 cells, to provide preliminary in vitro experiment reference for the treatment of bone metastasis of prostate cancer with oridonin. Methods The effect of oridonin on the proliferation of PC-3 cells was detected by CCK8. The effect of oridonin on the migration and invasion of PC-3 cells was detected by the Transwell test. The expression of miR-204-5p and NF-κB signaling pathways after the treatment of PC-3 cells with oridonin was analyzed by qRT-PCR. The expression of protein in the NF-κB signaling pathway after the treatment of PC-3 cells with oridonin was analyzed by Western blotting. Results Firstly, the proliferation of PC-3 cells was inhibited by oridonin solution in a concentration- and time-dependent manner; and the difference from the control group was of statistical significance (P<0.01), the IC50 value of oridonin was 10 μmol/L. Secondly, the migration and invasion of PC-3 cells were inhibited by oridonin in a concentration-dependent manner; and the difference from the control group was of statistical significance (P<0.01). Finally, the results of qRT-PCR showed that oridonin solution promoted the expression of miR-204-5p in PC-3 cells and blocked the expression of the downstream genes in the NF-κB signaling pathway; the results of Western blotting indicated that both the overexpression of miR-204-5p and oridonin could inhibit the expression of the downstream genes in NF-κB signaling pathway in PC-3 cells. Conclusions Oridonin can inhibit the proliferation, migration and invasion of PC-3 cells. Its mechanism of action may be associated with its up-regulating miR-204-5p and blocking the NF-κB signaling pathway.

Key words

Oridonin; / / Prostate cancer with bone metastasis; / / miR-204-5p; / / NF-κB signaling pathway

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Shi Haoran, Bao Guanglong, LingHu Xitao, Wu Honghan, Zhao Zeju, Liu Xiao, Fu Yao , Huang Shuai, Wa Qingde. Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p [J]. Chinese Journal of Clinical Anatomy. 2022, 40(3): 286-291 https://doi.org/10.13418/j.issn.1001-165x.2022.3.08

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