Establishment of the groin flap based on the superficial epigastric artery into axillary fossa in rats

Wu Dongfang, Liu Donghong, Fang Fang, Jing Xing, Chen Shaofeng, Zhuang Yuehong

Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (3) : 271-276.

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (3) : 271-276. DOI: 10.13418/j.issn.1001-165x.2022.3.06

Establishment of the groin flap based on the superficial epigastric artery into axillary fossa in rats

  • Wu Dongfang1, Liu Donghong2, Fang Fang3, Jing Xing3, Chen Shaofeng3, Zhuang Yuehong3*
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Abstract

Objective To describe the procedure of transfer of the groin flap based on the superficial epigastric artery into the axillary fossa in rats.   Materials   Twenty clean SD male rats weighing 420~450 g were depilated after successful anesthesia. A free groin flap model with a size of 4cm×2cm was established based on the superficial blood vessel of the abdominal wall was transplanted to the axillary fossa with the lateral thoracic vessels used as the recipient vessels. The main observations were as follows: ① the origin, course and branches of femoral vessels and lateral thoracic vessels were observed, and their outer diameter and length were measured; ② survival state of experimental animals; ③ The survival of the flap on the 7th day after operation.    Results    The diameter of the femoral artery and vein was (0.72±0.08) mm and (1.29±0.17) mm respectively, and that of the lateral thoracic artery and vein was (0.59±0.18) mm and (1.04±0.12) mm respectively. Two rats failed to recover from anesthesia after operation, and 3 rats had complete necrosis of skin flap, resulting in a flap survival rate of 75%. Due to removal of the femoral artery, claudication of the affected limb could occur after surgery.  One week later, with compensation of the blood supply, the gait of rats gradually returned to normal.    Conclusions   The free flap based on the superficial epigastric artery in rats is very suitable for junior doctors and researchers who want to engage in the clinical work and the basic research relating to supramicrosurgery.

Key words

Free flap /   /   / Superficial epigastric artery /   /   / Common thoracic artery /   /   / Vascular anastomosis 

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Wu Dongfang, Liu Donghong, Fang Fang, Jing Xing, Chen Shaofeng, Zhuang Yuehong. Establishment of the groin flap based on the superficial epigastric artery into axillary fossa in rats[J]. Chinese Journal of Clinical Anatomy. 2022, 40(3): 271-276 https://doi.org/10.13418/j.issn.1001-165x.2022.3.06

References

[1]  Kozusko SD, Liu X, Riccio CA, et al. Selecting a free flap for soft tissue coverage in lower extremity reconstruction[J].Injury, 2019, 50(5):S32-S39. DOI: 10.1016/j.injury.2019.10.045.
[2] Graboyes EM, Hornig JD. Evolution of the anterolateral thigh free flap[J].Curr Opin Otolaryngol Head Neck Surg, 2017, 25(5):416-421. DOI: 10.1097/MOO.0000000000000394.
[3]  徐永清,何晓清,陈雪松,等. 手和足部创面修复时四种吻合血管的皮神经营养血管穿支皮瓣的选择[J]. 中华显微外科杂志,2020,43(4):331-337. DOI:10.3760/ cma.j.cn441206-20200218-00068.
[4]  谭延曦, 潘志军,黄路,等. 内增压在修薄股前外侧穿支皮瓣游离移植修复四肢软组织缺损中的应用[J].中华显微外科杂志,2020,43(4):342-346. DOI:10.3760/cma.j.cn441206-20191129-00365. 
[5]  Plenter RJ, Grazia TJ. Murine heterotopic heart transplant technique[J].J Vis Exp, 2014, (89): 51511. DOI: 10.3791/51511.
[6]  Pichierri A, Frati A, Santoro A, et al. How to set up a microsurgical laboratory on small animal models:organization, techniques, and impact on residency training[J]. Neurosurg Rev, 2009, 32 (1):101-110. DOI: 10.1007/s10143-008-0154-4.
[7]  Edmunds MC, Wigmore S, Kluth D. In situ transverse rectus abdominis myocutaneous flap: a rat model of myocutaneous ischemiareperfusion injury[J]. J Vis Exp, 2013, (76): 50473. DOI: 10.3791/50473.
[8]  伊力扎提·伊力哈木, 朱辉, 刘爱蓬, 等. 超级显微外科血管吻合训练非活体血管模型的制备[J]. 中华显微外科杂志, 2020, 43(4): 368-370. DOI: 10.3760/cma.j.cn441206-20191125-00362.
[9]  Liu HL. Microvascular anastomosis of submillimeter vessels-a training model in rats[J]. J Hand Microsurg, 2013, 5(1): 14-17. DOI: 10.1007/s12593-013-0089-z.
[10] Lähteenmäki T, Waris T, Asko-Seljavaara S, et al. Recovery of sensation in free flaps[J]. Scandinavian J Plastic Reconstructive Surg, 1989, 23(3): 217-222. DOI: 10.3109/02844318909075121.
[11] Kerawala CJ, Newlands C, Martin I. Spontaneous sensory recovery in non-innervated radial forearm flaps used for head and neck reconstruction[J]. Int J Oral Maxillofac Surg, 2006, 35(8): 714-717. DOI: 10.1016/j.ijom.2006.03.014.
[2] Boyd B, Mulholland S, Gullane P,et al. Reinnervated lateral antebrachial cutaneous neurosome flaps in oral reconstruction: are we making sense[J]?  Plastic  Reconstr Surg, 1994, 93(7):1360-1362. DOI: 10.1097/00006534-199406000-00004.
[13] Mah SM, Durham JS, Anderson DW, et al. Functional results in oral cavity reconstruction using reinnervated versus nonreinnervated free fasciocutaneous grafts[J]. J Otolaryngol, 1996, 25(2): 75-78. PMID: 8683656.
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