Establishment of a new mechanical traction vasospasm model

doi:10.13418/j.issn.1001-165x.2022.1.13

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (1) : 67-71. DOI: 10.13418/j.issn.1001-165x.2022.1.13

Establishment of a new mechanical traction vasospasm model

Ma Qiming1, Cao Chenxin1, Chen Shaofeng1, Xie Yun2*

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Abstract

Objective　To establish a mechanical traction vasospasm model, for simulating vasospasm post-surgery. 　Methods　Forty-eight Sprague-Dawley rats were randomly divided into 6 groups: Group 1-5 was vascular traction groups (traction force was 5,10,15,20 and 25g, respectively); Group 6 was blunt vascular dissection group (control group). Laser Speckle contrast imaging was used to monitor the average blood flow perfusion, and to collect the duration of spasm and the time needed to achieve hyperperfusion as statistical parameters.　Results　Group 5 had hyperspasticity and was not included in the study. The duration and hyperperfusion time of vasospasm in group 1 and group 2 were relatively short, which was statistically significant compared with the control group (P<0.05). There was no significant difference between the third group and the control group (spasm duration P=0.801; hyperperfusion time P=0.629). Compared with the control group, the fourth group showed statistically significant difference in spasm duration (P=0.014), but no statistically significant difference in hyperperfusion duration (P=0.081). Conclusions　Vasospasm induced by bidirectional tension of 15g can well simulate clinical vascular dissection, and it is a reliable model with high success rate.

Key words

Mechanical vasospasm model / 　 Micro-reconstructive surgery / 　Myogenic vasospasm

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Ma Qiming, Cao Chenxin, Chen Shaofeng, Xie Yun. Establishment of a new mechanical traction vasospasm model[J]. Chinese Journal of Clinical Anatomy. 2022, 40(1): 67-71 https://doi.org/10.13418/j.issn.1001-165x.2022.1.13

References

[1] 徐达传, 钟世镇. 深入开展基础与临床研究促进皮瓣外科的进一步发展[J]. 中国临床解剖学杂志, 2004, 22(1): 3-4. DOI: 10.3969/j.issn.1001-165X.2004.01.001.
[2] 吴召森, 侯建玺, 谢书强, 等. 三定点和四定点褥式外翻血管吻合法在断指(肢)再植中的应用[J]. 中华显微外科杂志, 2019, 42(1): 75-77. DOI: 10.3760/cma.j.issn.1001-2036.2019.01.023.
[3] Ueda K, Harii K. Comparative study of topical use of vasodilating solutions[J]. Scand J Plast Reconstr Surg Hand Surg, 2003, 37(4): 201-207. DOI: 10.1080/02844310310016377.
[4] Puckett CL, Winters RR, Geter RK, et al. Studies of pathologic vasoconstriction (vasospasm) in microvascular surgery[J]. J Hand Surg Am, 1985, 10(3): 343-349. DOI: 10.1016/s0363-5023(85)80033-2.
[5] Wadström J, Gerdin B. Nervous influence on traumatic vasospasm in the rabbit ear artery[J]. Microsurgery, 1991, 12(2): 89-95. DOI: 10.1002/micr.1920120207.
[6] Vargas CR, Iorio ML, Lee BT. A systematic review of topical vasodilators for the treatment of intraoperative vasospasm in reconstructive microsurgery[J]. Plast Reconstr Surg, 2015, 136(2): 411-422. DOI: 10.1097/PRS.0000000000001431.
[7] Zhou Y, Liu Y, Hao Y, et al. The mechanism of botulinum A on Raynaud syndrome[J]. Drug Des Devel Ther, 2018, 12(2): 1905-1915. DOI: 10.2147/DDDT.S161113.
[8] Yavuz C, Callskan A, Karahan O, et al. Evaluation of the vasoplegic impact of papaverine in the rat aorta[J]. J Pak Med Assoc, 2014, 64(6): 660-663.
[9] Hýža P, Streit L, Schwarz D, et al. Vasospasm of the flap pedicle: the effect of 11 of the most often used vasodilating drugs. Comparative study in a rat model[J]. Plast Reconstr Surg, 2014, 134(4): 574e-584e. DOI: 10.1097/PRS.0000000000000570.
[10]Fang F, Liu MM, Xiao JT, et al. Arterial supercharging is Mmore beneficial to flap survival due to quadruple dilation of venules[J]. J Surg Res, 2020, 247(2): 490-498. DOI: 10.1016/j.jss.2019.09.056.
[11]Hýža P, Streit L, Schwarz D, et al. Vasospasm of the flap pedicle - the new experimental model on rat[J]. Acta Chir Plast, 2014, 56(1-2): 3-11.
[12]Bumbaširević M, Lešić A, Palibrk T, et al. Lower limb replantation: 27 years follow up[J]. Injury, 2020, 51(Suppl 4): S77-S80. DOI: 10.1016/j.injury.2020.02.113.
[13]冯仕明, 王爱国. 穿支皮瓣在下肢创面修复中的临床应用研究进展[J]. 中华解剖与临床杂志, 2015, (3): 272-275. DOI: 10.3760/cma.j.issn.2095-7041.2015.03.024.
[14]韩夫, 郑朝, 王洪涛, 等. 带阔筋膜股前外侧游离皮瓣修复头部鳞状细胞癌切除后硬脑膜缺损的效果[J]. 中华烧伤杂志, 2020, 36(3): 219-223. DOI: 10.3760/cma.j.cn501120-20190505-00222.
[15]Turin SY, Walton RL, Dumanian GA, et al. Current practices in the management of postoperative arterial vasospasm in microsurgery[J]. J Reconstr Microsurg, 2018, 34(4): 242-249. DOI: 10.1055/s-0037-1612601.
[16]Rubanyi GM. Endothelium-derived relaxing and contracting factors[J]. J Cell Biochem, 1991, 46(1): 27-36. DOI: 10.1002/jcb.240460106.
[17]Wingard CJ, Browne AK, Murphy RA. Dependence of force on length at constant cross-bridge phosphorylation in the swine carotid media[J]. J Physiol, 1995, 488 (Pt 3): 729-739. DOI: 10.1113/jphysiol.1995.sp021004.
[18]马启明, 庄跃宏. 皮瓣移植术后血管痉挛机制与解痉挛药物研究进展[J]. 中国临床解剖学杂志, 2020, 38(2): 231-234. DOI: 10.13418/j.issn.1001-165x.2020.02.027.