Protective effects of clioquinol on the hippocampal neuronal apoptosis and learning and memory functions of epileptic mice

doi:10.13418/j.issn.1001-165x.2022.1.07

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (1) : 33-38. DOI: 10.13418/j.issn.1001-165x.2022.1.07

Protective effects of clioquinol on the hippocampal neuronal apoptosis and learning and memory functions of epileptic mice

Dou Xiaona¹, Li Xia², Li Xinyu¹, Zhang Li^1,3*

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Abstract

Objective To study the protective effects of clioquinol on the hippocampal neuronal apoptosis and learning and memory functions of epileptic mice. Methods The epileptic mice model were built by pilocarpine in this study. Mice were divided into 3 groups: a control group (saline), an epilepsy group (pilocarpine and saline) and a clioquinol group (pilocarpine and clioquinol). Then the distribution of zinc in hippocampus was observed by autometallography. The morphological changes and the number of neurons were checked by Nissl staining. The expression of Bcl-2 and Bax protein were detected by immunoblotting. Enzyme linked immunosorbent assay was used to check the expression of caspases-9 and caspases-3 protein. The seizure times, seizure level and seizure duration were checked by behavioral observation. The learning and memory ability was shown by T maze and Y maze. Results In the clioquinol group, the distribution of zinc in hippocampus reduced significantly, the damage of hippocampal neurons was improved and the number of neurons increased, the expression of Bcl-2 protein increased, while the expression of Bax, caspases-9 and caspases-3 protein decreased. The ratio of Bcl-2 to Bax increased. The seizure times, seizure level and seizure duration decreased. The ability of learning and memory was improved (P<0.01). Conclusions Clioquinol can protect hippocampal neurons and improve the learning and memory functions of epileptic mice.

Key words

Clioquinol; / / Epilepsy; / / Hippocampus; / / Learning and memory

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Dou Xiaona, Li Xia, Li Xinyu, Zhang Li. Protective effects of clioquinol on the hippocampal neuronal apoptosis and learning and memory functions of epileptic mice[J]. Chinese Journal of Clinical Anatomy. 2022, 40(1): 33-38 https://doi.org/10.13418/j.issn.1001-165x.2022.1.07

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