Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway

doi:10.13418/j.issn.1001-165x.2021.06.012

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Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (6) : 680-685. DOI: 10.13418/j.issn.1001-165x.2021.06.012

Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway

Hu Sihan^1,2, Xue Yuan^1,2, Jin Yesheng², Zhang Yuanshu², Shi Qin¹, Rui Yongjun²

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Abstract

Objective　To investigate the effect of irisin on angiogenesis in human umbilical vein endothelial cell (HUVEC) under acute inflammatory state and its regulatory mechanism.　Methods　 (1)The effects of different concentrations (0.1, 1.0, 5.0, 10.0, 20.0 ng/ml) of irisin on HUVEC proliferation were detected by CCK-8; (2) Acute inflammatory state of HUVEC was induced by LPS. The effect of irisin on cell migration and tubular formation was studied by scratch and tube forming experiments. The gene expression of related cytokines IL-1β, IL-6, TNF-α and PDGF-BB was studied by RT-PCR; (3) The expressions of proteins Erk1/2, Jak and Stat3 in the JAK/STAT3 signaling pathway were detected by Western Blot.　Results　 (1) The change of concentrations of recombinant irisin did not affect the proliferation of HUVEC; (2) Irisin promoted cell migration and tubular formation of HUVEC in the state of acute inflammation, and inhibited the expression of inflammatory factors IL-1β, IL-6 and TNF-α in HUVEC in the state of acute inflammation, and increased the gene expression of tissue repair factor PDGF-BB; (3) Irisin inhibited LPS-induced activation of the JAK/STAT3 pathway.　Conclusions　Irisin suppresses JAK/STAT3 signaling pathway, relieves acute inflammatory state of HUVEC induced by LPS, and promotes angiogenesis.

Key words

/ Irisin / 　Ischemia reperfusion / 　HUVEC / 　LPS / 　JAK/STAT3

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Hu Sihan, Xue Yuan, Jin Yesheng, Zhang Yuanshu, Shi Qin, Rui Yongjun. Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway[J]. Chinese Journal of Clinical Anatomy. 2021, 39(6): 680-685 https://doi.org/10.13418/j.issn.1001-165x.2021.06.012

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