Effects of hydrogen sulfide on the autophagy and apoptosis induced by oxygen-glucose deprivation-reoxygenation in rat neuron

doi:10.13418/j.issn.1001-165x.2021.04.012

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Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (4) : 426-430. DOI: 10.13418/j.issn.1001-165x.2021.04.012

Effects of hydrogen sulfide on the autophagy and apoptosis induced by oxygen-glucose deprivation-reoxygenation in rat neuron

Jiang Wenwu^1,2, Hong Yue³, Wu Lixiang², Deng Lvhong⁴

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Abstract

Objective　To investigate the effects of exogenous hydrogen sulfide (H2S) on autophagy and injury induced by oxygen glucose deprivation/reoxygenation (OGD/R) in neurons, and to clarify the molecular mechanism of H2S attenuating cerebral ischemia-reperfusion injury in rats.　Methods　Rat neuronal PC12 cells were used as the research object. Classical OGD/R was used to induce cell damage. Sodium thiosulfate (NaHS), a donor of H2S, was used to observe the effect of H2S on OGD/R-induced mTOR, AMPK and its phosphorylated AMPK. In order to clarify the role of AMPK in the regulation of autophagy in PC12 cells by H2S, AMPK overexpression plasmid was used to observe the effect of H2S on the damage of PC12 cells.　Results　The OGD/R-treated PC12 cells had decreased mTOR phosphorylation level and enhanced AMPK activity, and NaHS pretreatment partially reversed the above changes. The overexpression of AMPK can abolish the inhibitory effect of NaHS on OGD/R-induced autophagy and cell damage. Conclusions　H2S can inhibit the autophagy of brain neurons during ischemia-reperfusion by inhibiting the activation of AMPK, and finally reduce neuronal damage.

Key words

/ Hydrogen sulfide / Autophagy / 　Oxygen-glucose deprivation-reoxygenation / 　Sodium hydrosulfide

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Jiang Wenwu, Hong Yue, Wu Lixiang, Deng Lvhong. Effects of hydrogen sulfide on the autophagy and apoptosis induced by oxygen-glucose deprivation-reoxygenation in rat neuron[J]. Chinese Journal of Clinical Anatomy. 2021, 39(4): 426-430 https://doi.org/10.13418/j.issn.1001-165x.2021.04.012

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