Mechanical stress promotes ASCs proliferation via negative regulation of Lamin A/C expression in expanded prefabricated adipose tissue&nbsp;

doi:10.13418/j.issn.1001-165x.2021.04.011

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Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (4) : 420-425. DOI: 10.13418/j.issn.1001-165x.2021.04.011

Mechanical stress promotes ASCs proliferation via negative regulation of Lamin A/C expression in expanded prefabricated adipose tissue

Ren Jing¹, Wan Lingling ^1，2, Lu Feng¹, Chen Xihang¹

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Abstract

Objective　To explore the regulatory effect of Lamin A/C on the mechanical sensitivity of adipose-derived stromal cells (ASCs) proliferation and the mechanism of the stress induced fat regeneration. Methods　A rat model of EPAT vacuum aspiration was established. Specimen were collected and prepared at 1, 4, 8 weeks after operation. And the expression of Lamins A/C were detected by Western blot and immunochemistry to analyze the correlation between the protein expression of Lamins A/C and the stress induced fat regeneration. The overexpression of Lamins A/C lentiviral vectors was constructed in vitro and transfected with ASCs. Static stretching of ASCs was performed in vitro, and the proliferation degree of ASCs was detected by Western blot and immunofluorescence to clarify the regulatory effect of Lamin A/C on the proliferation of ASCs.　Results　In vivo experiments showed that the proliferation of ASCs in EPAT of rats increased, meanwhile, the expression level of Lamin A/C in EPAT decreased significantly. There was no statistical difference in the expression of Lamin B1 and the expression of Lamin B2. In vitro experiments showed that overexpression of Lamin A/C could inhibit significantly the regulatory effect of static mechanical stress on the proliferation of ASCs in vitro. Conclusions　Lamin A/C is involved in the process of stress induced fat regeneration via negatively regulating the proliferation of ASCs.

Key words

Lamin A/C / 　Adipose-derived stromal cells（ASCs） / 　Mechanical stress / 　Expanded Prefabricated Adipose Tissue（EPAT）

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Ren Jing, Wan Lingling, Lu Feng, Chen Xihang. Mechanical stress promotes ASCs proliferation via negative regulation of Lamin A/C expression in expanded prefabricated adipose tissue [J]. Chinese Journal of Clinical Anatomy. 2021, 39(4): 420-425 https://doi.org/10.13418/j.issn.1001-165x.2021.04.011

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