The role of miR-206 on regulation of ERK1/2 signaling pathway by targeting Cx43 in rabbit steroid-induced avascular necrosis of femoral head model

doi:10.13418/j.issn.1001-165x.2021.02.008

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Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (2) : 154-160. DOI: 10.13418/j.issn.1001-165x.2021.02.008

The role of miR-206 on regulation of ERK1/2 signaling pathway by targeting Cx43 in rabbit steroid-induced avascular necrosis of femoral head model

Xi Yuan^1,2, Luo Gaobin¹，Wei Guiqing¹，Qin Wentao¹, Bo Zhandong¹

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Abstract

Objective　To explore the role of MicroRNA(miR)-206 on regulating of extracellular signal-regulated protein kinase(ERK) 1/2 signaling pathway by targeting connexin(Cx) 43 in rabbit model of steroid-induced avascular necrosis of femoral head.　Methods　Sixty mature rabbits were randomly divided into a model group (n=30) and a control group (n=30). Rabbit model of femoral head necrosis was made with lipopolysaccharide (LPS) and methylprednisolone (MPS). The establishment of the model was determined by MRI and HE staining after modeled 2、8 and 16 weeks. Total RNA and protein were extracted from the femoral head. In situ hybridization and Quantitative Real-time PCR (qPCR) were employed to detect the change of miRNA-206; qPCR, western blot and immunohistochemistry were used to detect the expression of Cx43、ERK1/2 and Runx2.　Results　The model success rate was 70%. In situ hybridization result showed that the miR-206 expressed in rabbit femoral head medullary space, osteoblasts and osteocyte. Compared with the control group, miR-206 expression of the model group up-regulated at 2 weeks, 8 weeks and 16 weeks after modeling, the expression of Cx43 and Runx2 mRNA in the model group down-regulated, the expression of Cx43、ERK1/2 and Runx2 protein in the model group also down-regulated.　Conclusions miR-206 can be involved in the occurrence, development and repair of steroid-induced femoral head necrosis by down-regulating its target protein CX43, inhibiting the ERK1/2 signaling pathway and osteogenic differentiation.

Key words

Femur head necrosis / 　miRNA-206 / 　Cx43 / 　ERK1/2

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Xi Yuan, Luo Gaobin, Wei Guiqing, Qin Wentao, Bo Zhandong. The role of miR-206 on regulation of ERK1/2 signaling pathway by targeting Cx43 in rabbit steroid-induced avascular necrosis of femoral head model[J]. Chinese Journal of Clinical Anatomy. 2021, 39(2): 154-160 https://doi.org/10.13418/j.issn.1001-165x.2021.02.008

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