The expression and localization of p-PDGFR alpha at different stages after substantia nigra striatum injury in mice

doi:10.13418/j.issn.1001-165x.2021.01.015

Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (1) : 76-81. DOI: 10.13418/j.issn.1001-165x.2021.01.015

Pei Dan, Liu Xue

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Abstract

Objective To investigate the expression and localization of platelet-derived growth factor receptor alpha (PDGFRα) at different stages after substantia nigra striatum injury in mice. Methods The substantia nigra striatum pathway was unilaterally transected in 8 weeks old BALB/c mice. According to the method of Baidan, brain tissues of 2 mm around the injury brain were examined on the 1st, 4th, 7th and 14th day after operation. The expression of p-PDGFR alpha protein was detected by immunohistochemical staining and Western blot. Immunofluorescence double staining was used to detect the localized expression of p-PDGFR alpha. Results Immunohistochemical staining and Western blot showed that the expression of p-PDGFR alpha reached its peak level 4 days after injury. Double immunofluorescence staining showed that p-PDGFR alpha was expressed in astrocytes, microglia, leukocytes and oligodendrocytes. Conclusions Brain injury can activate PDGFR alpha and express around the injury. Activated PDGFR alpha may be related to the proliferation of astrocytes, microglia, leukocytes and oligodendrocytes.

Key words

PDGFR / 　Brain injury;　Astrocytes;　Microglia / 　Oligodendrocytes / 　Leukocytes

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Pei Dan, Liu Xue. The expression and localization of p-PDGFR alpha at different stages after substantia nigra striatum injury in mice[J]. Chinese Journal of Clinical Anatomy. 2021, 39(1): 76-81 https://doi.org/10.13418/j.issn.1001-165x.2021.01.015

References

[1] Spencer T, Domeniconi M, Cao ZX, et al. New roles for old proteins in adult CNS axonal regeneration[J]. Curr Opin Neurobiol, 2003, 13(1): 133-139. DOI: 10.1016/s0959-4388(03)00012-6.
[2] Mocchetti I, Wrathall JR. Neurotrophic factors in central nervous system trauma[J]. J Neurotrauma, 1995, 12(5): 853-870. DOI: 0.1089/neu.1995.12.853.
[3] Kimura-Kuroda J, Teng X, Komura Y, et al. An in vitro model of the inhibition of axon growth in the lesion scar formed after central nervous system injury[J]. Mol Cell Neurosci, 2010, 43(2): 177-187. DOI: 10.1016/j.mcn.2009.10.008.
[4] Schachtrup C, Ryu JK, Helmrick MJ, et al. Fibrinogen triggers astrocyte scar formation by promoting the availability of active TGF-beta after vascular damage[J]. J Neurosci, 2010, 30(17): 5843-5854. DOI: 10.1523/JNEUROSCI.0137-10.2010.
[5] Hayashi Y, Nomura M, Yamagishi S, et al. Induction of various blood-brain barrier properties in non-neural endothelial cells by close apposition to co-cultured astrocytes[J]. Glia, 1997, 19(1): 13-26. PMID: 8989564.
[6] 白丹, 裴丹, 刘囡, 等. 降解纤维蛋白原可减少创伤性脑损伤后胶质瘢痕与纤维瘢痕的形成[J]. 解剖学报, 2014, 45(6): 729-734. DOI: 10.3969/j.issn.0529-1356.2014.06.001.
[7] Strbian D, Durukan A, Tatlisumak T. Rodent models of hemorrhagic stroke[J]. Curr Pharm Des, 2008, 14(4): 352-358. DOI: 10.2174/138161208783497723.
[8] Wang Z, Kong D, Banerjee S, et al. Down-regulation of platelet-derived growth factor-D inhibits cell growth and angiogenesis through inactivation of Notch-1 and nuclear factor-kappaB signaling[J]. Cancer Res, 2007, 67(23): 11377-11385. DOI: 10.1158/0008-5472.CAN-07-2803.
[9] Hoch RV, Soriano P. Roles of PDGF in animal development[J]. Development, 2003, 130(20): 4769-4784. DOI: 10.1242/dev.00721.
[10]Li R, Maminishkis A, Wang FE, et al. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines[J]. Invest Ophthalmol Vis Sci, 2007, 48(12): 5722-5732. DOI: 10.1167/iovs.07-0327.
[11]Li X, Kumar A, Zhang F, et al. VEGF-independent angiogenic pathways induced by PDGF-C[J]. Oncotarget, 2010, 1(4): 309-314. DOI: 10.18632/oncotarget.141.
[12]Uutela M, Wirzenius M, Paavonen K, et al. PDGF-D induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis[J]. Blood, 2004, 104(10): 3198-3204. DOI: 10.1182/blood-2004-04-1485.
[13]Deuel TF, Senior RM, Huang JS, et al. Chemotaxis of monocytes and neutrophils to platelet-derived growth factor[J]. J Clin Invest, 1982, 69(64): 1046-1049. DOI: 10.1172/jci110509.
[14]Thommen R, Humar R, Misevic G, et al. PDGF-BB increases endothelial migration on cord movement during angiogenesis in vitor[J]. J Cell Biochem, 1997, 64(3): 403-413. PMID: 9057098.
[15] Kumagai S, Ohtani H, Nagai T, et al. Platelet-derived growth factor and its receptors are expressed in areas of both active inflammation and active fibrosis in inflammatory bowel disease[J]. Tohoku J Exp Med, 2001, 195(1): 21-33. DOI: 10.1620/tjem.195.21.
[16] Miyatake SI, Furuse M, Kawabata S, et al. Bevacizumab treatment of symptomatic pseudoprogression after boron neutron capture therapy for recurrent malignant gliomas. Report of 2 cases[J]. Neuro-oncology, 2013, 15(6): 650-655. DOI: 10.1093/neuonc/not020.
[17] Ungvari Z, Podlutsky A, Sosnowska D, et al. Ionizing radiation promotes the acquisition of a senescence-associated secretory phenotype and impairs angiogenic capacity in cerebromicrovascular endothelial cells: role of increased DNA damage and decreased DNA repair capacity in microvascular radiosensitivity[J]. J Gerontol Series A Biol Sci Med Sci, 2013, 68(12): 1443-1457. DOI: 10.1093/gerona/glt057.