Effect of miR-146a on the sensitivity of nasopharyngeal carcinoma cells HNE-1 / DDP to cisplatin by targeting CCNJ

doi:10.13418/j.issn.1001-165x.2021.01.011

Chinese Journal of Clinical Anatomy ›› 2021, Vol. 39 ›› Issue (1) : 55-59. DOI: 10.13418/j.issn.1001-165x.2021.01.011

Zhou Lanzhu, Zhao Bao, Zhang Mingjie, Cui Yixuan, Wu Jun, Sun Zhe, Liu Shencheng, Wang Wenzhong

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Abstract

Objective　To investigate the effect of miR-146a on cisplatin sensitivity of nasopharyngeal carcinoma HNE-1 / DDP cells and its possible mechanism.　Methods　The experiment was divided into three groups: a blank control group, a negative control group and a mimics-miR-146a group. MTT assay was used to detect the proliferation inhibition of cells after different treatments. Annexin V-FITC / PI assay was used to detect apoptosis. Western blot was used to detect the relative expression levels of cyclin CCNJ, MRP1, P-gp. RT-qPCR was used to detect the relative expression level of CCNJ mRNA.　Results Compared with other two groups, the survival rate, the relative protein expression levels of MRP1, P-gp, CCNJ and the relative expression level of CCNJ mRNA of mimics-miR-146a reduced, while the apoptosis rate increased. There were statistical differences in the survival rate, the relative protein expression levels of MRP1, P-gp, CCNJ, the relative expression level of CCNJ mRNA and the apoptosis rate among the three groups (P<0.05).　Conclusions　miR-146a can increase the sensitivity of HNE-1/DDP to DDP which mechanism may be through miR-146a inhibiting the expression of CCNJ to regulate downstream proteins MRP1,P-gp,and then to reduce cell resistance.

Key words

Nasopharyngeal carcinoma / 　Drug resistance / 　Micro RNA / 　Cisplatin / 　Cyclin protein

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Zhou Lanzhu, Zhao Bao, Zhang Mingjie, Cui Yixuan, Wu Jun, Sun Zhe, Liu Shencheng, Wang Wenzhong. Effect of miR-146a on the sensitivity of nasopharyngeal carcinoma cells HNE-1 / DDP to cisplatin by targeting CCNJ[J]. Chinese Journal of Clinical Anatomy. 2021, 39(1): 55-59 https://doi.org/10.13418/j.issn.1001-165x.2021.01.011

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