Effect of TRPC6 inhibitor SAR7334 on biological behavior of cervical cancer&nbsp;

doi:10.13418/j.issn.1001-165x.2020.06.010

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (6) : 674-679. DOI: 10.13418/j.issn.1001-165x.2020.06.010

Effect of TRPC6 inhibitor SAR7334 on biological behavior of cervical cancer

JIAN Meng-chan¹, FAN Li2, WU Yun³, SHI Dan-dan³, LU Min³, LIU Rui³, HE Xi-ju^3,4

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Abstract

Objective　To investigate the expression of TRPC6 in cervical cancer and the effect of its inhibitor SAR7334 on the proliferation and migration of Siha （Human cervical squamous cell carcinoma cell）.　Methods　Eleven cases of cervical squamic carcinoma diagnosed in People’s hospital of Shiyan from September 2017 to September 2019 were selected as the experimental group. Normal cervical tissue of the same patient was used as the control group. Western-blot and immunofluorescence were used to detect the expression of TRPC6; Siha cells were treated with 20, 100, 1000 nmol/L SAR7334, cell proliferation and apoptosis were detected by CCK8 method and Hoechst staining. Cell migration was detected by scratch test and Transwell test. After TRPC6 channel was blocked, the change of autophagy in Siha cells was detected by immunoblotting.　Results　The expression of TRPC6 protein in cervical cancer tissues was significantly higher than that of normal tissues. Compared with the control group, after blocking TRPC6 with 100 nmol/L SAR7334, the proliferation and migration of Siha cells reduced, the scratch healing rate reduced, and the apoptosis rate increased (P<0.05). After the TRPC6 channel was blocked, the autophagy of Siha cell weakened.　Conclusions　The expression of TRPC6 is up-regulated in cervical cancer, and its inhibitor SAR7334 may inhibit the proliferation and migration of cervical cancer Siha cells by affecting cell autophagy.

Key words

/ TRPC6；　SAR7334；　Cervical cancer Siha cells；　Autophagy

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JIAN Meng-chan, FAN Li, WU Yun, SHI Dan-dan, LU Min, LIU Rui, HE Xi-ju. Effect of TRPC6 inhibitor SAR7334 on biological behavior of cervical cancer [J]. Chinese Journal of Clinical Anatomy. 2020, 38(6): 674-679 https://doi.org/10.13418/j.issn.1001-165x.2020.06.010

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