Objective To investigate the effect of cutting vagus nerve hepatic branch on blood glucose and lipid levels and its relationship with mTOR expression level in rats, to provide experimental evidence for the study of neuromodulation mechanism of glycolipid metabolism. Methods Rat model of hepatic branch of vagus nerve transection was established by surgical method (n=15), and the sham operation group (n=15) with vagus hepatic branch exploration and the control group without any treatment (n=15) were set up. The levels of blood glucose, blood lipid, insulin and cortisol, glycogen content in liver and skeletal muscle and the expression of mTOR were measured every two weeks after operation. Results Compared with the control group, two weeks after operation, the blood glucose level of the experimental group decreased, the content of liver glycogen increased and the level of insulin increased (P<0.05). Six weeks after operation, the level of blood glucose increased, the content of glycogen in liver and skeletal muscle decreased, the level of triglyceride increased, the level of HDL decreased, and the expression of mTOR in liver and skeletal muscle increased (P<0.05). There was no significant difference between the sham group and the control group at each time point (P>0.05). Conclusions The transetion of rat vagus nerve hepatic branch can lead to abnormal glucose and lipid metabolism and glycogen content, which may be related to the changes in insulin level and increased expression of mTOR in liver and skeletal muscle.
Key words
Hepatic branch of vagus nerve /
Rat; Metabolism /
Glycogen /
mTOR
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