Digital holographic microscopy (DHM) and its application in monitoring three-dimensional dynamics of human spermatozoa

doi:10.13418/j.issn.1001-165x.2020.04.021

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (4) : 476-480. DOI: 10.13418/j.issn.1001-165x.2020.04.021

SUN Li¹, HUANG Gui², GONG Xiang-jun² , OU Jian-ping¹

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Abstract

Objective　To apply digital holographic microscopy (DHM) as a tool for monitoring three-dimensional (3D) dynamics of human spermatozoa.　Methods　Sperm samples collected from healthy male participants were diluted to 2×107/mL by artificial human tubal fluid (HTF). These suspensions were injected for further observations. 3D dynamics of human spermatozoa were recorded by DHM with holograms. Background images were obtained by averaging holograms in each subgroup. Background removal was performed and the back-free hologram was applied for 3D localization of the sperm according to Rayleigh-Sommerfeld back propagation algorithm. Instantaneous 3D coordinates of human spermatozoa were linked to form trajectories. Subsequently, 3D motion patterns, density distribution, spatial distribution of 3D velocity and 3D orientation of human spermatozoa were determined.　Results　3D motility parameters, 3D motion patterns as well as information reflecting sperm-surface interactions including density distribution, spatial distribution of 3D velocity and 3D orientation of human spermatozoa were available by utilizing DHM. Conclusions　DHM can provide a comprehensive assessment of sperm fertilizing capacity.

Key words

Digital holographic microscopy / 　Human spermatozoa / 　Three-dimensional dynamics

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SUN Li, HUANG Gui, GONG Xiang-jun , OU Jian-ping. Digital holographic microscopy (DHM) and its application in monitoring three-dimensional dynamics of human spermatozoa[J]. Chinese Journal of Clinical Anatomy. 2020, 38(4): 476-480 https://doi.org/10.13418/j.issn.1001-165x.2020.04.021

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