The chronic stress induced expression of DPP-4 and its role in adipose inflammation and glucose metabolism

doi:10.13418/j.issn.1001-165x.2020.04.014

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (4) : 438-443. DOI: 10.13418/j.issn.1001-165x.2020.04.014

ABULAIKEMU·Mahemuti¹, MAIMAITI·Yisireyili^2,3, AIKEBAIER·Aili^2,3, LI Yi-liang³, AZIGULI -Alimujiang², YAN Jing³, KELIMU·Abudureyimu^2,3

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Abstract

Objective　To analyze chronic stress induced dipeptidyl peptidase-4 (DPP-4) expression and its role in adipose inflammation and glycometabolism.　Methods　20 male SPF mice were randomly divided into two groups: a stress group and a control group. Mice in the stress group were restrained in our own restraint device for 2 hours per day and lasted for 14 days. The expression of macrophage surface marker (CD11b) in adipose tissue was detected by immunohistochemistry. The expression of DPP-4, adipocytokines (Adiponectin, MCP-1, IL-6, TNF-α) in adipose tissue and the expressions of glucose metabolism molecules (IRS-1, GLUT-4) were further detected by RT-PCR. The enzymatic activity of DPP-4 and the concentration of GLP-1 were detected by ELISA.　Results　The white adipose tissue (WAT) of the stress group mice significantly retracted and WAT weight significantly reduced (P<0.001). WAT in the stress group showed a large number of monocytes, neutrophils infiltration and inflammatory changes. Mice in the stress group significantly decreased the expression of Adiponectin and significantly increased the expression of MCP-1, IL-6 and TNF-α (P<0.001). DPP-4 expression in WAT tissues and its activity in blood increased significantly in the stress group, but the concentration of serum GLP-1 decreased significantly (P<0.001). The mRNA level of IRS-1 and mRNA level of GLUT-4 in WAT of the stress group were significantly lower than that of the control group (P<0.001). Conclusions　Chronic stress induced abnormal expression of DPP-4 in adipose tissue, which can lead to adipose inflammation and abnormal glycometabolism.

Key words

Chronic stress / 　Dipeptidyl peptidase-4 / 　Adipocytokine / 　Inflammation / Glycometabolism

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ABULAIKEMU·Mahemuti, MAIMAITI·Yisireyili, AIKEBAIER·Aili, LI Yi-liang, AZIGULI -Alimujiang, YAN Jing, KELIMU·Abudureyimu. The chronic stress induced expression of DPP-4 and its role in adipose inflammation and glucose metabolism[J]. Chinese Journal of Clinical Anatomy. 2020, 38(4): 438-443 https://doi.org/10.13418/j.issn.1001-165x.2020.04.014

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