Effects of tea polyphenol&nbsp; on peripheral nerve injury and repair

doi:10.13418/j.issn.1001-165x.2020.03.012

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (3) : 301-307. DOI: 10.13418/j.issn.1001-165x.2020.03.012

Effects of tea polyphenol on peripheral nerve injury and repair

TANG Yin-juan¹, CHEN Long², DUAN Yan-zhi², LI Shu², ZHU Bin², ZUO Zhi-min², WANG Xiang-hai³, LI Zhen-lin³

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Abstract

Objective 　To explore the effects of tea polyphenol (TP) on peripheral nerve injury and repair.　Methods　48 adult BALB/c mice were randomly divided into three groups: a TP treatment group, a saline control group and a sham group. The right sciatic nerve of each mouse in the TP treatment group and the saline control group was injured by crush and then the mice were intragastrically administrated with TP or saline twice per day for successive 14 days. The mice in sham group were only exposed their right sciatic nerve without other treatment. The motor function of each mouse was detected by footprint assay and the sensory function was evaluated with acupuncture assessment 14 days after the surgery. Subsequently, the sciatic nerves of 6 mice in each group were collected for qPCR assay. The other 10 mice of each group were subjected to perform the NF-200 / MBP double immunostaining to show the regenerated axons and myelin in the distal trunk of the injured nerves. The gastrocnemius muscle was weighed and then to prepare the HE stained section.　Results　Compared with the saline control group, the TP treatment resulted in significantly improved functions of motor and sensory, higher wet weight ratio of gastrocnemius muscle and bigger area size of cross-section of myofibers, as well as more regenerated axons and myelin in the injured nerves. Conclusions　This study show that tea polyphenols could improve the behavioral functional loss and neuropathologic changes caused by nerve injury.

Key words

Tea polyphenol (TP） / 　Periphral nerve injure / 　Nerve regeneration

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TANG Yin-juan, CHEN Long, DUAN Yan-zhi, LI Shu, ZHU Bin, ZUO Zhi-min, WANG Xiang-hai, LI Zhen-lin. Effects of tea polyphenol on peripheral nerve injury and repair[J]. Chinese Journal of Clinical Anatomy. 2020, 38(3): 301-307 https://doi.org/10.13418/j.issn.1001-165x.2020.03.012

References

[1] Ma F, Xiao Z, Chen B, et al. Linear ordered collagen scaffolds loaded with collagen-binding basic fibroblast growth factor facilitate recovery of sciatic nerve injury in rats[J]. Tissue Eng Part A, 2014, 20(7-8): 1253-1262.
[2] Höke A. A (heat) shock to the system promotes peripheral nerve regeneration[J]. J Clin Invest, 2011, 121(11): 4231-4234.
[3] Amniattalab A, Mohammadi R. Functional, histopathological and immunohistichemical assessments of cyclosporine a on sciatic nerve regeneration using allografts: a rat sciatic nerve model[J]. Bull Emerg Trauma, 2017, 5(3): 152-159.
[4] Niedzwiecki A, Roomi MW, Kalinovsky T, et al. Anticancer efficacy of polyphenols and their combinations[J]. Nutrients, 2016, 8(9): E552.
[5] 李淑娟. 茶多酚的保健和药理作用研究进展[J]. 西北药学杂志, 2010, 1(25): 78-79.
[6] Mandel S, Weinreb O, Amit T, et al. Cell signaling pathways in the neuroprotective actions of the green tea polyphenol (-)-epigall　ocatechin-3-gallate: implications for neurodegenerative diseases[J]. J Neurochem, 2004, 88(6): 1555-1569.
[7] Weinreb O, Amit T, Mandel S, et al. Neuroprotective molecular mechanisms of (-)-epigallocatechin-3-gallate: a reflective outcome of its antioxidant, iron chelating and neuritogenic properties[J]. Genes Nutr, 2009, 4(4): 283-296.
[8] Ehrnhoefer DE, Bieschke J, Boeddrich A, et al. EGCG redirects amyloidogenic polypeptides into unstructured, off-pathway oligomers[J]. Nat Struct Mol Biol, 2008, 15(6): 558-566.
[9] Li W, Zhu S, Li J, et al. EGCG stimulates autophagy and reduces cytoplasmic HMGB1 levels in endotoxin-stimulated macrophages[J]. Biochem Pharmacol, 2011, 81(9): 1152-1163.
[10] Kim HS, Montana V, Jang HJ, et al. Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells[J]. J Biol Chem, 2013, 288(31): 22693-22705.
[11] Renno WM, Al-Khaledi G, Mousa A, et al. (-)-Epigallocatechin-3-gallate (EGCG) modulates neurological function when intravenously infused in acute and, chronically injured spinal cord of adult rats[J]. Neuropharmacology, 2014, 77: 100-119.
[12] 张晓梦, 倪艳, 李先荣. 茶多酚的药理作用研究进展[J]. 药物评价研究, 2013, 36(2): 157-160.
[13] 蒋勤. 茶多酚的药理作用[J].中国药师, 2006(1): 63-64.
[14] Williams RJ, Spencer JP. Flavonoids, cognition, and dementia: actions, mechanisms, and potential therapeutic utility for Alzheimer disease[J]. Free Radic Biol Med, 2012, 52(1): 35-45.
[15] Xue R, Wu G, Wei X, et al. Tea polyphenols may attenuate the neurocognitive impairment caused by global cerebral ischemia/reperfusion injury via anti-apoptosis[J]. Nutr Neurosci, 2016, 19(2): 63-69.
[16] Kitagawa N, Morikawa T, Motai C, et al. The antiproliferative effect of chakasaponins I and II, floratheasaponin A, and epigallocatechin 3-O-Gallate isolated from camellia sinensis on human digestive tract carcinoma cell lines[J]. Int J Mol Sci, 2016, 17(12): E1979.
[17] 杨渐, 俞昌喜. 周围神经损伤的药物治疗进展[J]. 中国实用神经疾病杂志, 2010, 13(11): 91-92,51.
[18] 路青林, 刘庆胜, 李志, 等. 神经节苷脂神经生长因子促进周围神经再生的实验研究[J]. 生物骨科材料与临床研究, 2007, 4(2): 40-42.
[19] Baluchnejadmojarad T, Roghani M. Chronic oral epigallocatechin-gallate alleviates streptozotocin-induced diabetic neuropathic hyperalgesia in rat: involvement of oxidative stress[J]. Iran J Pharm Res, 2012, 11(4): 1243-1253.
[20]Lin LC, Wang MN, Tseng TY, et al. Pharmacokinetics of (-)-epigallocatechin-3-gallate in conscious and freely moving rats and its brain regional distribution[J]. J Agric Food Chem, 2007, 55(4): 1517-1524.
[21] Nakagawa K, Miyazawa T. Absorption and distribution of tea catechin, (-)-epigallocatechin-3-gallate, in the rat[J]. J Nutr Sci Vitaminol, 1997, 43(6): 679-684.
[22] 赵亚男, 柯永胜, 郑青山, 等. 茶多酚对家犬的长期毒性作用[J]. 中国临床药理学与治疗学, 2000, 5(1): 43-46.
[23]Lee JS, Kim YT, Jeon EK, et al. Effect of green tea extracts on oxaliplatin-induced peripheral neuropathy in rats[J]. BMC Complement Altern Med, 2012, 12: 124.
[24] 陈一菁, 王祥海, 潘梦婕, 等. PLGA复合有序多孔胶原蛋白支架制备人工神经修复大鼠坐骨神经缺损[J]. 中华神经医学杂志, 2017, 16(8): 757-765.