Objective　To investigate the morphological characteristics of the cubital fossa perforators, providing anatomical basis for restoring the antecubital defects by using the V-Y advancement flap based on the cubital fossa perforators.　Methods　30 sides of adult cadaveric upper extremities were collected to perform red latex perfusion, macro-micro-anatomy, and mimic operation. The following contents were observed under a surgical magnifier: ①The morphology of the cubital fossa; ②The origin, branches, and distribution of the cubital fossa perforators. ③The anastomosis of the cubital fossa perforators and the peripheral rete  vasculosum.　Results　①The cubital fossa was a triangle lacuna in the anterior aspect of the elbow, which was a passing portal for antebrachial vessels and nerves. ②The blood supply for the skin of the cubital fossa was multi-source, which was commonly composed of the brachial artery and the perforators from the branches of the brachial artery. The perforators had a large variation and could be classified into three types according to the number and outer diameter: A. Thick and concentrated type (13/30, accounting for 43.33%): this type had 1 perforator (inferior cubital artery) with an average outer diameter of 0.6~1.2 mm. B. Tiny and dispersive type (11/30, accounting for 36.67%): this type had 3~4 perforators with an average outer diameter of 0.3~0.6 mm; C. Moderate and balanced type (6/30, accounting for 20%): this type had 2 perforators with an average outer diameter of 0.4~0.7 mm; ③ The perforators punctured out of the deep fascia along either side of the cutaneous antebrachial lateralis nerve or cephalic vein towards the antecubital skin, which also anastomosed with each other and with adjacent vessels to form the fascial vascular network of cubital fossa.　Conclusions　The V-Y advancement flap based on the cubital fossa perforators could become a good supplement for restoring the antecubital defects.