Spermidine alleviates acute lung injury induced by lipopolysaccharide in mice

doi:10.13418/j.issn.1001-165x.2020.01.013

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (1) : 62-66. DOI: 10.13418/j.issn.1001-165x.2020.01.013

SU Qing¹, CAO Li², PENG Zhuo², LI Qi ², YI Liu-xiang², GUO Xian-xian², LIU Fang-ting², SUN Guo-ying¹

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Abstract

Objective To observe the effects of spermidine on acute lung injury (ALI) induced by lipopolysaccharide (LPS). Methods ALI mice model was established by intratracheal drip of LPS ( 5 mg/kg ) . Effects of spermidine on respiratory function of ALI mice were detected by small animal ventilator, the effects of spermidine on lung tissue morphology of ALI mice were observed. The total protein, total cell number and neutrophil number in bronchoalveolar lavage fluid (BALF) of ALI mice were detected, and the level of myeloperoxidase (MPO) was tested. The expression of TREM-1 mRNA in lung tissue of ALI mice was detected by qPCR, and the protein level of sTREM-1 in BALF of ALI mice was measured by ELISA. Results Spermidine could improve the respiratory function of ALI mice, alleviate LPS-induced lung pathological damage, decrease protein exudation and neutrophil infiltration, and reduce the expression of TREM-1, an inflammatory amplification receptor in the lungs of ALI mice. Conclusions Spermidine may reduce the infiltration of inflammatory cells, inhibit the expression of inflammatory factors and alleviate LPS-induced ALI. The mechanism may be related to the inhibition of TREM-1 expression in lung tissue of ALI mice by spermidine.

Key words

Spermidine / 　Acute lung injury / 　Lipopolysaccharide / 　Triggering receptor expressed on myeloid cells-1

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SU Qing, CAO Li, PENG Zhuo, LI Qi, YI Liu-xiang, GUO Xian-xian, LIU Fang-ting, SUN Guo-ying. Spermidine alleviates acute lung injury induced by lipopolysaccharide in mice[J]. Chinese Journal of Clinical Anatomy. 2020, 38(1): 62-66 https://doi.org/10.13418/j.issn.1001-165x.2020.01.013

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