Studying the effect of miR-382 on renal fibrosis in high glucose based on 3D culture system of renal mesangial cells

doi:10.13418/j.issn.1001-165x.2020.01.010

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (1) : 45-50. DOI: 10.13418/j.issn.1001-165x.2020.01.010

LI Xiang¹, YANG Zhi-feng², WANG Guo-bao¹

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Abstract

Objective　To explore the effect of miR-382 in diabetic nephropathy and renal fibrosis by constructing 3D renal mesangial cells spheroid with LBL.　Methods　LBL was utilized to construct 3D renal mesangial cells spheroid for studying the effect of different concentrations of glucose on renal mesangial cells and the effect of miR-382 on it. The experimental groups were divided into three groups according to the concentrations of glucose (5.6 mmol/L, 25 mmol/L, 40 mmol/L). qRT-PCR, Western blotting and immunofluorescence were used to detect the expression of TGF-β1, Fn, miR-382 and the level of its possible target gene the 10th chromosome deficiency phosphatase and tension of homologous gene (PTEN).　Results High glucose upregulated the expression of miR-382, downregulated secretion of PTEN, and significantly increased the expression of TGF-β1 and Fn.　Conclusions 　miR-382 may be involved in the pathology lesion of mesangial cells in the early state of diabetic nephropathy and renal fibrosis via negatively regulating the expression of PTEN.

Key words

LBL;　3D;　High glucose;　miR-382 / 　Renal fibrosis

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LI Xiang, YANG Zhi-feng, WANG Guo-bao. Studying the effect of miR-382 on renal fibrosis in high glucose based on 3D culture system of renal mesangial cells[J]. Chinese Journal of Clinical Anatomy. 2020, 38(1): 45-50 https://doi.org/10.13418/j.issn.1001-165x.2020.01.010

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