Objective To observe the expression of PRD1-BF1-RIZ1 homologous domain containing 16 (PRDM16), uncoupling protein 1 (UCP1), fatty acid synthase (FAS) and acetyl Coenzyme A carboxylase (ACC) in dopamine D2 receptor knockout mice. Methods The body weight and fat mass of wild type group (WT) and dopamine D2 receptor knockout group (D2KO) were compared. Real-time quantitative RT-PCR and Western blotting analysis were used to detect PRDM16, UCP1, FAS and ACC expression in BAT of both groups. Results Body weight of the WT group was (23.78±0.54) g while D2KO group was (20.54±1.15) g. The epididymal adipose tissue (eAT) fat mass was (1.083±0.02) g while the D2KO group was (0.978±0.03) g. The subcutaneous adipose tissue (sAT) fat mass was (1.85±0.05) g while the D2KO group was (1.72±0.03) g. The brown adipose tissue (BAT) fat mass was (31.38±4.22) mg while the D2KO group was (42.44±2.47) mg. Compared with the WT group, the mRNA and protein expression of UCP1 in BAT were significantly decreased in D2KO group while the mRNA and protein expression of PRDM16, FAS, ACC were significantly increased. The mRNA and protein level of ACC did not change between two groups. Conclusion The expression of PRDM16, UCP1, FAS and ACC in BAT may be related to the weight loss of dopamine D2 receptor knockout mice. Compared with the WT group, the mRNA and protein expression of UCP1 in BAT is significantly decreased in D2KO group while the mRNA and protein expression of PRDM16, FAS, ACC is significantly increased.
Key words
Dopamine D2 receptor knockout mice /
Weight loss /
Brown adipose tissue
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