Establishment of acquired tracheoesophageal fistula by ultra-minimally invasive magnetic compression technique in rabbits
GAO Hui-min1,2,3, QIU Ming-long2,3, ZHANGg Yan-chao2,3, LIU Hao2,3, MA Si-jie2,3, FU Shan 2,3, LV Yi1,2, YAN Xiao-peng1,2
Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (2) : 223-227.
Establishment of acquired tracheoesophageal fistula by ultra-minimally invasive magnetic compression technique in rabbits
Objective To explore the feasibility of establishing ultra-invasive magnetic compression technology to establish a rabbit model of tracheal esophageal fistula(TEF). Methods Ten New Zealand rabbits were anesthetized and inserted into the parent magnet and daughter magnet in the cervical trachea and esophagus, respectively. X-ray was taken the following day to observe the location of the mother and daughter magnets. When the parent and daughter magnets were found to be out of the original position and entered the digestive tract, the animals were sacrificed three days later. The general specimens of tracheal esophagus were taken, and the tissue of the fistula was taken for HE and Masson staining for pathological observation. Results The average operation time of magnetic compression ultra-invasive technique to establish a TEF was (3.20 ± 0.81) min. (6.90 ± 1.14) d after operation the magnets fell into the digestive tract, while TEF formatted. There was a slight adhesion between the tracheal membrane and the anterior wall of the esophagus, and the tissue around the fistula was observed. Histopathological observation showed that the stenosis was connected to the anterior wall of the esophagus, which was closely related to the pathological features of the clinically acquired tracheoesophageal fistula. Conclusions Establishment of rabbit TEF using magnetic compression ultra-minimally invasive technology is a simple, minimally invasive, and feasible method for animal model of TEF. It can be used as an ideal animal modeling method for obtaining TEF.
Magnetic compression technique / Tracheoesophageal fistula / Animal model / Minimal invasive
[1] 王伟, 陈永卫, 孙海梅. 先天性食管闭锁并气管食管瘘的大鼠胚胎学研究[J]. 中华小儿外科杂志, 2009, 30(4): 242-245.
[2] Santosham R. Management of acquired benign tracheoesophageal fistulae[J]. Thorac Surg Clin, 2018, 28(3): 385-392.
[3] Scappaticci E, Ardissone F, Baldi S, et al. Closure of an iatrogenic tracheo-esophageal fistula with bronchoscopic gluing in a mechanically ventilated adult patient[J]. Ann Thorac Surg, 2004, 77(1): 328-329.
[4] 程庆书, 韩勇, 刘锟, 等. 获得性气管食管瘘的诊治[J]. 中华消化外科杂志, 2007, 6(6): 410-412.
[5] 白慧健, 马太芳, 李钊. 先天性食管闭锁并气管食管瘘的早期胚胎学研究[J]. 河北医科大学学报, 2011, 32(7): 755-757.
[6] 杨光, 白光振, 李小飞, 等. 获得性食管气管瘘食管改道术后旷置食管结构及屏障功能研究[J]. 医学研究杂志, 2010, 39(5): 70-74.
[7] 严小鹏, 刘雯雁, 李涤尘, 等. 消化外科手术的内镜化途径:磁吻合联合内镜[J]. 世界华人消化杂志, 2014, 22(19): 2716-2721.
[8] An Y, Zhang Y, Liu H, et al. Gastrojejunal anastomosis in rats using the magnetic compression technique[J]. Sci Rep, 2018, 8(1): 11620.
[9] Yan X, Fan C, Ma J, et al. Portacaval shunt established in six dogs using magnetic compression technique[J]. PLoS One, 2013, 8(9): e76873.
[10]Ellebaek MBB, Qvist N, Rasmussen L. Magnetic compression anastomosis in long-gap esophageal atresia gross type a: a case report[J]. European J Pediatr Surg Rep, 2018, 6(1): e37-e39.
[11] Yan XP, Zou YL, She ZF, et al. Magnet compression technique: a novel method for rectovaginal fistula repair[J]. Int J Colorectal Dis, 2016, 31(4): 937-938.
[12] 严小鹏, 任冯刚, 刘雯雁, 等. 磁锚定技术联合磁压榨技术实现超微创胃造瘘[J]. 中华实验外科杂志, 2016, 33(2): 291-294.
/
| 〈 |
|
〉 |
