Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion
CHEN Chuan-qi, CHEN Long-ju, WU Tai-ding, TAN Yun-xia, LI Dan, WANG Yu
Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (2) : 148-152.
Effect of Puerarin on the expression of MMP-9 protein in spinal cord after brachial plexus root avulsion
Objective To investigate the expression changes of matrix metalloproteinase-9 (MMP-9) in spinal cord after brachial plexus root avulsion and the effect of Puerarin on its expression. Methods A total of 174 adult male SD rats were randomly divided into a normal group, a model group, Puerarin low, medium and high treatment groups. The model group and the Puerarin treatment groups underwent the right C5~C7 anterior nerve root avulsion. The rats were injected intraperitoneally Puerarin every day after the operation. The expression of MMP-9 protein in C5~C7 segment of spinal cord was detected by Western Blot. Results Compared with the normal group, the expression of MMP-9 protein peaked at the 1st day after brachial plexus root avulsion, decreased near to normal at the 3rd day and decreased to a lower level after 1 week. The expression of MMP-9 protein in spinal cord tissue of Puerarin treatment group was significantly lower than the model group at the 1st day. Conclusion MMP-9 may be involved in the early injury response to brachial plexus root avulsion, and Puerarin may help to alleviate this injury.
Puerarin / Brachial plexus / Nerve injury / Matrix metalloproteinase-9
[1] Li W, Wang S, Zhao J, et al. Complications of contralateral C-7 transfer through the modified prespinal route for repairing brachial plexus root avulsion injury: a retrospective study of 425 patients[J]. J Neurosurg, 2015, 122(6): 1421-1428.
[2] Vanaclocha V, Herrera JM, Verdu-Lopez F, et al. Transdiscal C6-C7 contralateral C7 nerve root transfer in the surgical repair of brachial plexus avulsion injuries[J]. Acta Neurochir (Wien), 2015, 157(12): 2161-2167.
[3] Teles RM, Antunes SL, Jardim MR, et al. Expression of metalloproteinases (MMP-2, MMP-9, and TACE) and TNF-alpha in the nerves of leprosy patients[J]. J Peripher Nerv Syst, 2007, 12(3): 195-204.
[4] Chattopadhyay S, Myers RR, Janes J, et al. Cytokine regulation of MMP-9 in peripheral glia: implications for pathological processes and pain in injured nerve[J]. Brain Behav Immun, 2007, 21(5): 561-568.
[5] Shiying L, Xinhui Q, Guanghua J, et al. Puerarin promoted proliferation and differentiation of dopamine-producing cells in Parkinson's animal models[J].Biomed Pharmacother, 2018, 106: 1236-1242.
[6] Liu B, Zhao C, Li H, et al. Puerarin protects against heart failure induced by pressure overload through mitigation of ferroptosis[J]. Biochem Biophys Res Commun, 2018, 497(1): 233-240.
[7] Xie HT, Xia ZY, Pan X, et al. Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury[J]. Neural Regen Res, 2018,13(7): 1263-1268.
[8] Chen L J, Ren YH, Liu L, et al. Upregulated expression of GAP-43 mRNA and protein in anterior horn motoneurons of the spinal cord after brachial plexus injury[J]. Arch Med Res, 2010, 41(7): 513-518.
[9] Zhong Y, Huang YL, Hu YM, et al. Puerarin alleviate radicular pain from lumbar disc herniation by inhibiting ERK-dependent spinal microglia activation[J].Neuropeptides, 2018, 72: 30-37.
[10]Inciong JG, Marrocco WC, Terzis JK, et al. Efficacy of intervention strategies in a brachial plexus global avulsion model in the rat[J]. Plast Reconst Surgr, 2000, 105(6): 2059-2071.
[11] Zhou LH, Wu W. Survival of injured spinal motoneurons in adult rat upon treatment with glial cell line-derived neurotrophicfactor at 2 weeks but not at 4 weeks after root avulsion[J]. J Neurotrauma, 2006, 23(6): 920-927.
[12]陈传奇. 葛根素对臂丛根性撕脱后脊髓前角运动神经元NGF、GAP-43mRNA及蛋白表达的影响[D]. 恩施: 湖北民族学院, 2017: 20-25.
[13] 盛华均, 孙善全. c-myc、MMP-9在受压静脉管壁的表达[J]. 中国临床解剖学杂志, 2009, 27(4): 448-451.
[14] 张同星, 井万里, 张涛, 等. 神经调节素对缺血再灌注损伤大鼠脊髓MMP-9和TIMP-1表达的影响[J]. 中国脊柱脊髓杂志, 2016, 26(7): 642-649.
[15]Zhang P, Ma X. Effect of rutin on spinal cord injury through inhibition of the expression of MIP-2 and activation of MMP-9, and downregulation of Akt phosphorylation[J]. Mol Med Rep, 2015, 12(5): 7554-7560.
[16] Yu F, Kamada H, Niizuma K, et al. Induction of mmp-9 expression and endothelial injury by oxidative stress after spinal cord injury[J]. J Neurotrauma, 2008, 25(3): 184-195.
[17]Bakos SR, Costanzo RM. Matrix metalloproteinase-9 is associated with acute inflammation after olfactory injury[J]. Neuroreport, 2011, 22(11): 539-543.
[18]Girard S, Sébire G, Kadhim H. Proinflammatory orientation of the interleukin 1 system and downstream induction of matrix metalloproteinase 9 in the pathophysiology of human perinatal white matter damage[J]. J Neuropathol Exp Neurol, 2010, 69(11): 1116-1129.
[19]周华, 刘华. 脊髓损伤后MMP-9 表达的时空分布及其意义[J]. 创伤外科杂志, 2005, 7(3): 175-177.
[20]Gundogdu EB, Bekar A, Turkyilmaz M, et al. CDP-choline modulates matrix metalloproteinases in rat sciatic injury[J]. J Surg Res, 2016, 200(2): 655-663.
[21]Tanaka T, Moriyama T, Kawamura Y, et al. Puerarin Suppresses Macrophage Activation via Antioxidant Mechanisms in a CaPO4-Induced Mouse Model of Aneurysm[J]. J Nutr Sci Vitaminol (Tokyo), 2016, 62(6): 425-431.
[22]Saraswat G, Guha R, Mondal K, et al. Molecular cues to the anti-implantation effect of nano-puerarin in rats[J]. Reproduction, 2016, 151(6): 693-707.
[23]Tian F, Xu LH, Zhao W, et al. The neuroprotective mechanism of puerarin treatment of acute spinal cord injury in rats[J]. Neurosci Lett, 2013, 543: 64-68.
/
| 〈 |
|
〉 |
