Effects of forsythoside on oxidative stress and renal function in aged rats
YANG Jie-ling, LI Chen, LIU Qing, HUANG Qiu-yan, FENG Ying
Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (1) : 77-82.
Effects of forsythoside on oxidative stress and renal function in aged rats
Objective To investigate the effects and related mechanisms of forsythoside on oxidative stress and renal function in aged rats. Methods Fifteen SD female aged rats (24 months old) were randomly divided into three groups with 5 rats in each: the old control group (Aged rats), the low dose forsythia group [Aged rats+FA (20 mg/kg)] and the high dose forsythia group [Aged rats+FA (100 mg/kg)]. Another 5 female SD rats (12 weeks old) served as the young control group (Young rats). Rats in low and high dose forsythiaside groups were administered for 60 days by gavage. Coomassie brilliant blue was used to detect the total protein of 24 h urine. And the levels of serum creatinine and urea nitrogen were detected by related kits. Renal injury was detected by HE staining and TUNEL staining. Western Blot was used to detect the expression of Caspase-3 and Caspase-9. The levels of SOD, MDA and GSH were detected by related kits. ELISA was used to detect the levels of inflammatory cytokines IL-18 and IL-1 beta in serum. Results Compared with the young control group, the content of protein in urine increased significantly in the aged control group. The content of creatinine and urea nitrogen in the serum increased significantly. The arrangement of renal tissue cells was irregular. A large number of inflammatory cells were infiltrated. The ratio of apoptotic cells increased significantly. The expression level of Caspase-3 and Caspase-9 increased significantly. The activity of SOD decreased significantly. MDA content increased significantly. GSH content decreased significantly. Serum IL-18 and IL-1 beta increased significantly. The expression of Nrf 2, HO-1 and NQO-1 decreased significantly（P<0.01）. In the low and high dose group, the content of protein in urine decreased significantly. The content of creatinine and urea nitrogen in the serum decreased significantly. The arrangement of renal tissue cells tended to be regular. The infiltration of inflammatory cells decreased significantly. And the ratio of apoptotic cells decreased significantly. The expression level of Caspase-3 and Caspase-9 decreased significantly. The activity of SOD increased significantly. The content of MDA decreased significantly. The content of GSH increased significantly. The contents of IL-18 and IL-1 beta in serum decreased significantly. The expression of Nrf 2, HO-1 and NQO-1 increased significantly（P<0.01）. Conclusions Forsythin inhibits oxidative stress and improves renal function in aged rats，and the mechanism may be related to the activation of Nrf 2-ARE pathway.
Forsythiaside / Oxidative stress / Kidney function / Apoptosis / Nrf 2-ARE
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