Role of calpain-1 in the inhibition of simvastatin on myocardial inflammation in diabetic rats

doi:10.13418/j.issn.1001-165x.2019.01.013

Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (1) : 60-63. DOI: 10.13418/j.issn.1001-165x.2019.01.013

HAN Qian-qian ^1,2, LU Mei-li ¹, WANG Hong-xin¹, TANG Fu-tian¹ , ZHANG Ying-jie²

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Abstract

Objective　To investigate the role of calpain-1 in the inhibition of simvastatin on myocardial inflammation in diabetic rats.　Methods　Forty SD rats were randomly divided into 4 groups: blank control group (CON group), streptozotocin group (STZ group), simvastatin high dose (20 mg/kg) treatment group (STZ+SIM20 group) and low dose (10 mg/kg) treatment group (STZ+SIM10 group). After 12 weeks, the pathological change of myocardial tissue was observed by HE staining. The expression of p65 was detected by immunohistochemistry. The expression of calpain-1, p65 and IκBα was detected by Western Blot. Serum TNF-α, IL-6 content was detected by ELISA.　Results　Compared with the STZ group, the SIM treatment group had neatly arranged cardiomyocytes and decreased inflammatory cell infiltration. The expression of p65 and IκBα protein in the cytoplasm was increased, and the expression of calpain-1, TNF-α, IL-6 and nucleus p65 in the nucleus was decreased.　Conclusion　Simvastatin has a protective effect on myocardial inflammation in diabetic rats, and its mechanism may be related to the inhibition of myocardial calpain-1 expression.

Key words

Simvastatin / 　Diabetic rats / 　Calpain-1 / 　Inflammation

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HAN Qian-qian, LU Mei-li, WANG Hong-xin, TANG Fu-tian, ZHANG Ying-jie. Role of calpain-1 in the inhibition of simvastatin on myocardial inflammation in diabetic rats[J]. Chinese Journal of Clinical Anatomy. 2019, 37(1): 60-63 https://doi.org/10.13418/j.issn.1001-165x.2019.01.013

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