Triggering receptor expressed on myeloid cells-1 inhibits autophagy of macrophages under LPS stress
ZHU Ai-ping, LIANG Dao-miao, SU Qing, LI Li-fang, JIA Zhe, HE Li-ping, REN Xiang, SUN Guo-ying
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (6) : 642-647.
Triggering receptor expressed on myeloid cells-1 inhibits autophagy of macrophages under LPS stress
Objective To observe the effect of TREM-1 on the expression of autophagy related genes in macrophages. Methods The expression of TREM-1 protein in macrophages exposed to lipopolysaccharide (LPS) was observed. TREM-1 agonists (MAB1187) and TREM-1 antagonists (LR12) were used to regulate the activities of macrophage. qPCR was used to detect macrophage autophagy related protein ATG7, ATG5, ATG12, and microtubule associated protein 1 light chain 3 (LC3) mRNA, respectively. Expression of TREM-1, LC3 II/I, and ATG7 protein were detected by Western Blot. The expression of autophagy marker protein LC3 in macrophages under LPS stress and TREM-1 activation was detected by immunofluorescence. Results The expression of TREM-1 protein in macrophages was significantly increased under the stress of LPS (400 ng/mL, 1000 ng/mL), and the TREM-1 protein expression in macrophages reached a peak when LPS (1000 ng/mL) acted as macrophage 24 h. ATG7, ATG5, ATG12 and LC3 mRNA expression of macrophage were reduced by LPS stress. When TREM-1 antagonists were given, the expression of ATG7, ATG5, ATG12 and LC3 mRNA increased, and the autophagic gene expression of macrophage was reversed after the use of TREM-1 activator. Western Blot assay showed that TREM-1 inhibited the expression of LC3 II/I and ATG7 proteins in macrophages under LPS stress. Immunofluorescence assay showed that TREM-1 could reduce the content of LC3 protein in macrophages. Conclusions The macrophage autophagy is inhibited after the activation of TREM-1 on the membrane of macrophage, suggesting that the TREM-1 of macrophage under LPS stress may play an inflammatory magnification by inhibiting the normal autophagy of macrophages.
/ TREM-1 / Macrophage / LPS / Autophagy
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