The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit
HAN Jian-long, QIU Si-qiang, ZHU Xin-wei, LUAN Fang-hai, CHANG Gang, CHENG Ji-zu, ZUO Jin-liang
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (6) : 621-625.
The study of neuropeptide Y in neural pathway between cervical spinal ganglia and cervical sympathetic ganglia in rabbit
Objective To study the neural pathway between cervical spinal ganglia and cervical sympathetic ganglia, and to explore the neural factors in the pathogenesis of cervical vertigo by detecting the neuropeptide Y (NPY) in cervical sympathetic ganglia after the cervical spinal ganglia electro-stimulated. Methods 96 New Zealand rabbits were divided into C2, C3, C4, C5, C6, C7 and control groups randomly (n=8 in each group ). The electro-stimulations (width=0.5 ms, 30 Hz, 5 V, time=5 s, 1 time/1 min, and 5 times total) were given to specific spinal ganglia which were revealed. After 30 min, the rabbits were killed through perfusion and the superior cervical sympathetic ganglia (SCG) and inferior cervical sympathetic ganglia (ICG) of both sides were cut. Then the section were made after immunologic reaction and observed under the microscope. Results The content of NPY in the same side sympathetic ganglia was higher than the other side sympathetic ganglia and the control group sympathetic ganglia after the electro-stimulation was given to the cervical spinal ganglia, and the difference was statistic significance (P<0.05). When the electro-stimulation was given to the C2, C3 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG mainly; when the electro-stimulation were given to the C4, C5 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in SCG and ICG; when the electro-stimulation were given to the C6, C7 spinal ganglia, the change of the NPY content of sympathetic ganglia were found in ICG mainly. Conclusion There is some kind of neural pathway between the cervical spinal ganglia and cervical sympathetic ganglia, and the characteristic of segment and homonymy in this kind of connection is also found, which provides a neuroanatomical basis for the hypothesis of reflex arc in cervical vertigo.
Cervical spinal ganglion / Cervical ganglion / Neuropeptide Y / Cervical vertigo / Nervous reflex arc
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