The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus
LIAO Pan，HE Yi，YANG Fang-yuan，ZHUANG Jian，LUO Gui-hu，ZHAI Ze-qing，SUN Er-wei
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (5) : 532-535.
The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus
Objective Evidence has demonstrated that the formation of neutrophil extracellular traps (NETs) formation mediated by neutrophil plays an important role in the pathogenesis and progression of systemic lupus erythematosus (SLE). In this study, we aimed to investigate whether reduced glutathione (GSH) could decrease the level of intracellular reactive oxygen species (ROS) or inhibit NETs formation. Methods Neutrophils were isolated from peripheral blood of SLE patients by gradient centrifugation method dextran sedimentation and centrifugation, and then were stimulated with phorbol 12-myristate 13-acetate (PMA). The production of intracellular ROS and the formation of NETs were examined. Results Compared with the PMA group treated with PMA, the group preprocessing with GSH could markedly suppress formation of NET by neutrophils from SLE patients. Moreover, the production of intracellular ROS production was significantly decreased in GSH-treated neutrophils from SLE patients. Conclusion GSH can significantly inhibit the process of neutrophils' NETosis from SLE patients through modulating the intracellular ROS production. These results indicates that GSH might be a potential therapeutic agent for SLE.
Reduced glutathione / Systemic lupus erythematosus / Neutrophils / NETosis / Reactive oxygen species
[1] Brinkmann V, Reichard U, Goosmann C, et al. Neutrophil extracellular traps kill bacteria[J]. Science, 2004, 303(5663):1532-1535.
[2] Papayannopoulos V. Neutrophil extracellular traps in immunity and disease[J]. Nat Rev Immunol, 2018, 18(2):134-147.
[3] Jorch SK, Kubes P. An emerging role for neutrophil extracellular traps in noninfectious disease[J]. Nat Med, 2017, 23(3):279-287.
[4] 谢雅清，梁晓美，叶伟霞. 还原型谷胱甘肽的药理作用与临床应用研究进展[J]. 中国药业, 2013, 22(7):124-127.
[5] 张北平，刘树锋，王海，等. GSH对于系统性红斑狼疮治疗观察[J]. 中国现代药物应用, 2016, 10(13):189-190.
[6] 张均，赵景宏，刘力，等. 还原型谷胱甘肽辅助治疗对狼疮肾炎患者肾小球系膜细胞CXCL9/10/11表达的影响[J]. 中国医学前沿杂志(电子版), 2017, 9(11):118-121.
[7] Domingo-Gonzalez R, Martinez-Colon G J, Smith A J, et al. Inhibition of neutrophil extracellular trap formation after stem cell transplant by prostaglandin E2[J]. Am J Respir Crit Care Med, 2016, 193(2):186-197.
[8] Lood C, Blanco LP, Purmalek MM, et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease[J]. Nat Med, 2016, 22(2):146-153.
[9] Hakkim A, Furnrohr BG, Amann K, et al. Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis[J]. Proc Natl Acad Sci U S A, 2010,107(21):9813-9818.
[10] Knight JS, Subramanian V, O'Dell AA, et al. Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL/lpr mice[J]. Ann Rheum Dis, 2015, 74(12):2199-2206.
[11] Knight JS, Zhao W, Luo W, et al. Peptidylarginine deiminase inhibition is immunomodulatory and vasculoprotective in murine lupus[J]. J Clin Invest, 2013, 123(7):2981-2993.
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