The effect of elevated shear stress on collateral vessel growth of aged rats
DONG Li-ping,LIU Guang-min, WANG Jia-jie, CAI Wei-jun
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (5) : 527-531.
The effect of elevated shear stress on collateral vessel growth of aged rats
Objective To investigate the effect of elevated shear stress on collateral vessel growth of aged rats by comparing the model induced by femoral artery ligation with femoral arteriovenous anastomosis and collateral vessel growth model with simple femoral artery ligation. Methods 30 aged (24 months) rats were divided into sham group, ligation group and arteriovenous anastomosis group. The rats in the ligation group underwent femoral artery ligation, and the rats in the anastomosis group were subjected to femoral artery ligation and femoral arteriovenous anastomosis. The animals survived for 1 week after surgery. 5 rats in each group were taken to isolate the muscle with collateral vessels of the hindlimb for immunohistochemical for detecting expression of eNOS, Ki67 and MMP2; the other 5 rats were used for angiography, after which X-ray photography was performed to observe the growth of collateral vessels. Results Compared with the sham operated group, the number of collateral vessels increased in the ligation group (P<0.01), the number of Ki67-positive cells increased (P<0.01), and so as the expression of eNOS (P<0.001) and MMP2 (P<0.05). Compared with the ligation group, the number of collateral vessels in femoral artery ligation with femoral arteriovenous anastomosis group was increased（P<0.01）, the number of Ki67-positive cells increased significantly (P<0.01), which was the same with the expression of eNOS (P<0.001) and MMP2 (P<0.01). Conclusion Elevated shear stress can promote the growth of collateral vessels in the ischemic hindlimb of aged rats.
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