Architecture of periodontal capillary casting in Wistar rat periodontitis model
LAI Qiu-hua, ZHANG Jie-xin, ZHOU Xiao-han, WANG Ya-jie, LI Ye, LIU Yang, BI Zhen-yu, OUYANG Jun
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (4) : 423-425.
Architecture of periodontal capillary casting in Wistar rat periodontitis model
Objective To build the periodontal capillaries casting of Wistar rats by perfusion of their capillaries with microgranular materials, which provided a morphological method for study the microcirculation of periodontitis. Methods 8-week-old Wistar rats were used to establish the animal model of periodontitis. Micropump was implanted from the inlet of the left ventricle of the aorta, and the liquid of the microgranular material was perfused to rely on the pressure of the pump. The swelling and redness of the skin indicated the success of perfusion. After the filling material was fully polymerized in the specimen for 24 hours, the soft tissue was removed by corrosion. The capillary cast specimens of periodontitis were observed with the S-3000N scanning electron microscope (SEM). Results Under scanning electron microscope from 30, 2000, until 4500 times, the casting of the periodontal capillary, the three-dimensional space morphology and distribution of the capillaries of structures such as the gingival margin, gingival crevicular, gingival papillae could be clearly viewed in the casting of the periodontal capillary. Conclusion The capillary cast can provide the morphological methods for periodontitis microcirculation research, and can be coordinated with the molecular biologic research of periodontitis, revealing various pathological changes of periodontitis from different aspects.
[1] Krechina EK, Zorina OA, Molchanov AM, et al. Microcirculation impairment in periodontal tissues in patients with chronic generalized periodontitis combined with metabolic syndrome[J]. Stomatologiia (Mosk), 2016, 95(1): 27-30.
[2] Boillot A, Bouchard P, Moss K, et al. Periodontitis and retinal microcirculation in the Atherosclerosis Risk in Communities study[J]. J Clin Periodontol, 2015, 42(4): 342-349.
[3] Funaki S, Tokutomi F, Wada-Takahashi S, et al. Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP) [J]. Microb Pathog, 2016, 92:36-42.
[4] 李忠华, 黄潮桐, 刘阳, 等. 用自凝牙托材料制作软体解剖标本[J]. 中国临床解剖学杂志, 2007, 25(5): 611.
[5] 童建斌, 黄菊芳, 王慧, 等. 明胶墨汁与荧光微球显示大鼠视网膜微血管灌流效果的对比研究[J]. 中国临床解剖学杂志, 2008, 26(6): 655-658.
[6] Matheus ASM, da Matta MFB, Clemente ELS, et al. Sensibility and specificity of laser speckle contrast imaging according to Endo-PAT index in type 1 diabetes[J]. Microvasc Res, 2017, 117:10-15.
[7] Nakanishi K, Fukuda S, Tanaka A, et al. Relationships between periventricular epicardial adipose tissue accumulation, coronary microcirculation, and left ventricular diastolic dysfunction[J]. Can J Cardiol, 2017, 33(11):1489-1497.
[8] Plotnikov MB, Aliev OI, Sidekhmenova AV, et al. Dihydroquercetin improves microvascularization and microcirculation in the brain cortex of SHR rats during the development of arterial hypertension[J]. Bull Exp Biol Med, 2017, 163(1): 57-60.
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