The regulation of hypoxic induced factor on autophagy of myocardial cells in oxygen-glucose deprivation model
SONG Hai-yan, LIAN Hui, ZHANG Yi-min, FU Sheng-qi
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (4) : 419-422.
The regulation of hypoxic induced factor on autophagy of myocardial cells in oxygen-glucose deprivation model
Objective To investigate the effect of HIF-1α on autophage-associated genes Beclin1, LC3I and LC3II, and the regulation on autophagy. Methods The model of oxygen-glucose deprivation (OGD) was established, and western blot was used to detect interference effect of HIF-1α siRNA. The CCK-8 assay was used to detect the effect of HIF-1α siRNA on the activity of myocardial cells in OGD model. The effect of HIF-1α siRNA on autophagy-associated genes Beclin1, LC3I and LC3II expression was detected by RT-qPCR and western blot. Results The results of western blot showed that HIF-1α siRNA could effectively knock down the expression of HIF-1α in myocardial cells in OGD model. The result of CCK-8 assay showed that HIF-1α siRNA could reduce the activity of myocardial cells in OGD model. RT-qPCR and western blot results showed that HIF-1α siRNA could reduce the expression of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA and protein level. Conclusion HIF-1α siRNA could knock down the expression of HIF-1α in the OGD model, resulting in further decrease of the activity of myocardial cells，with the Beclin1 expression and ratio of LC3II/LC3I reduced. HIF-1α plays a role of myocardial protection in the OGD model by regulation of autophagy.
Ischemia / Hypoxia / Autophagy; Hypoxic induction factor / Myocardial injury
[1] Semenza GL. Hypoxia-inducible factor 1 and cardiovascular disease[J]. Annu Rev Physiol, 2014, 76: 39-56.
[2] Nishida Y, Arakawa S, Fujitani K, et al. Discovery of Atg5 /Atg7-independent alternative macroautophagy [J]．Nature, 2009, 461(7264): 654-658.
[3] Przyklenk K, Undyala VV, Wider J, et al. Acute induction of autophagy as a novel strategy for cardioprotection: getting to the heart of the matter[J]. Autophagy, 2011, 7(4): 432-433.
[4] Semenza GL. Hypoxia-inducible factors in physiology and medicine[J]. Cell, 2012, 148(3): 399-408.
[5] Castino R, Isidoro C, Murphy D. Autophagy-dependent cell survival and cell death in an autosomal dominant familial neurohypophyseal diabetes insipidus in vitro model[J]. FASEB J, 2005, 19 (8): 1024-1026.
[6] Ghavami S, Gupta S, Ambrose E, et al. Autophagy and heart disease: implications for cardiac ischemia-reperfusion damage[J]. Curr Mol Med, 2014, 14(5): 616-629.
[7] Martins-Marques T, Ribeiro-Rodrigues T, Pereira P, et al. Autophagy and ubiquitination in cardiovascular diseases[J]. DNA Cell Biol, 2015, 34 (4): 243-251.
[8] Shintani T, Klionsky DJ. Autophagy in health and disease: a double-edged sword[J]. Science, 2004, 306(5698):990-995.
[9] Wu H, Huang S, Chen Z, et al. Hypoxia-induced autophagy contributes to the invasion of salivary adenoid cystic carcinoma through the HIF-1alpha/BNIP3 signaling pathway[J]. Mol Med Rep, 2015, 12(5):6467-6474.
[10] 王路乔，黄波，黄茶花，等. 自噬抑制剂促进缺氧诱导的大鼠乳鼠心肌细胞凋亡[J]. 基础医学与临床，2014, 34(2): 185-189.
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