Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization
PENG Zhang-song, HE Yun-fan, LU Feng
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (4) : 392-396.
Prepare decellularized adipose tissue by freeze-thaw cycle and mechanical homogenization
Objective Decellularized adipose tissue (DAT) was prepared by physical homogenization to improve its ability of spontaneous lipid induction. Methods In this study, we used homogenization method to remove lipid from adipose tissue, a novel DAT (DAT-1) was fabricated after subsequent decellularization process. Ordinary DAT served as control. Results The results revealed that DAT-1 achieved the same decellularization efficiency, but exhibited better retention of ECM components (laminin and collagen Ⅳ) and in vivo recellularization, compared to those with ordinary DAT. Conclusions Mechanical homogenization is a rapid and efficient method to remove lipid from adipose tissue, which could avoid the use of harsh chemical agent and lead to the better retention of ECM components, promoting the in vivo adipose tissue regeneration of DAT.
Decellularized matrix / Freeze-thaw cycle / Mechanical homogenization / Adipose tissue regeneration
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