Changes of MCT2 expression in cerebral cortex of rats with acute heart failure
SONG Kai-qin1, KANG Cheng-qiao2, SUN Shan-quan3
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (3) : 308-312.
Changes of MCT2 expression in cerebral cortex of rats with acute heart failure
Objective To observe the changes of monocarboxylate transporters 2( MCT2) expression in the rat cerebral cortex under the stimulation of acute heart failure syndrome, so as to detect the alterations of energy metabolism of the cerebral cortex under the condition of acute heart failure, and to provide experimental basis for the best rescue time of acute heart failure. Methods The model was established by intravenous injection of 1.5% pentobarbital sodium.To estimate whether acute heart failure model was successful or not, the left ventricular systolic pressure（LVSP）and increased rate of left ventricular pressure maximum（+dp/dt max）were recorded. Immunohistochemical staining(IHC) and Western blot(WB) were used to explore the alteration of MCT2 expression in rat cerebral cortex. Results Compared with the normal group, the number and the integral optical density（IOD）of MCT2-positive neurons were increased in the cerebral cortex of the acute heart failure model，reaching the highest level on 5 min and declined on 10 min, but still higher than those in the control group (P<0.05). Conclusion Our results involved that MCT2 is enhanced under the case of acute heart failure, which might indicate that MCT2 is involved in the process of self-compensation in the absence of cerebral ischemia.
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