HuR induces bone marrow mesenchymal stem cells into chondrogenic differentiation
LI Yong-jin, WU Hui-mei, LIN Yong-peng, HOU Yu,CHEN Bo-lai
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (3) : 304-308.
HuR induces bone marrow mesenchymal stem cells into chondrogenic differentiation
Objective To observe the effect of HuR on chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and to explore its mechanism. Methods BMSCs were extracted from 4-week-old SD rats. The third generation BMSCs were divided into a HuR group, and a NC group, and were respectively treated with pAdEasy-HuR-GFP virus solution and pAdEasy-GFP virus solution. After intervention, the cells were fixed 14 days after induction, paraffin-embedded and sectioned. Toluidine blue staining, QPCR and IHC detection of XIAP, HuR, Col2a1 expression differences were performed. Results Toluidine blue staining showed that positive signals of proteoglycan staining by HuR staining were more than NC and cartilage ball diameter was larger than NC. QPCR revealed that cartilage HuR, XIAP was significantly increased, Col2a1 up-regulation was not obvious. Immunohistochemical results showed that XIAP, HuR, Col2a1 gradually increased with the increase of drug-containing serum concentration, XIAP upward trend more obvious, HuR and Col2a1 upregulation is not obvious. QPCR detected cartilage HuR, XIAP was significantly increased, Col2a1 up-regulation is not obvious. Conclusion Overexpression of HuR facilitates the differentiation of BMSCs into cartilage, which may be related to the regulation of XIAP transcription after HuR over-expression.
RNA binding protein; HuR; / BMSC / Differentiation into cartilage
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