Conductive and elastic cryogel for enhancing the proliferation of C2C12 cell
HUANG Shi-jia，WU Wei-le，CHEN Xiao-xing，WANG Le-yu，LIU Cheng-long
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (3) : 277-281.
Conductive and elastic cryogel for enhancing the proliferation of C2C12 cell
Objective In this study, the mussle-inspired polypyrrole (Ppy)-methacrylated gelatin (GelMA) cryogel（DOPA-base Ppy-GelMA） was investigated as a scaffold to enhance the proliferation of C2C12 cell. Methods The material structure and cell adhesion were investigated by scanning electron microscopy. The influence of cryogel on C2C12 cells was confirmed by live/dead staining, CCK-8 test and Ki-67 immunofluorescence staining. Result The Ppy-GelMA cryogel had the uniform and interconnected porous structure. Interestingly, myoblasts in Ppy-GelMA cryogel were oriented aligned. Both the GelMA cryogel and the Ppy-GelMA cryogel were suitable for the attachment and spread of C2C12 myoblasts, while the cell viability in Ppy-GelMA hcryogel was significantly better than that in GelMA cryogel. Furthermore, the Ki67 positive myoblasts rate in Ppy-GelMA cryogel was significantly better than that in GelMA cryogel. Conclusion This Ppy-GelMA cryogel can enhance the proliferation of C2C12 myoblasts.
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