Objective　The origin, course and distribution of deep neurovascular structures in the dorsal side of the wrist were dissected to explore the relationship between neurovascular injury and wrist dorsal pain, and to provide anatomical basis for clinical diagnosis and treatment of wrist dorsal pain.　Methods　Under the head-mounted magnifying glass, the origin, couse and distribution of deep neurovascular structures in the dorsal side of 10 cadavers were studied on 20 sides of the upper limbs, and the relevant data were measured.　Results　The deep dorsal nerve of the wrist derived from the terminal branch of the posterior interosseous nerve, traveled through the fourth osseofibrous tunnel, and then distributed to the dorsal side of the wrist. The artery  derived from the terminal branch of the anterior interosseous artery. The anterior interosseous artery went through the anterior interosseous membrane of the arm to the back of the forearm. the terminal branch divided into the radial and ulnar branch, went through the fourth and fifth osseofibrous canal, respectively and distributed to the dorsal side of the wrist. The radial branch was associated with the posterior interosseous nerve endings in the fourth osseofibrous canal. The ulnar branch passed alone through the fifth osseofibrous canal.    Conclusion　It is of great significance to prevent, diagnose and treat pain of dorsal wrist by having a thorough understanding of the anatomy of deep neurovascular structures in dorsal aspect of the wrist.