Study on the protective effect of Tongxinluo on kidneys of diabetic rats based on the expression of TGF- β1 and CTGF
LIU Ying, ZHANG Xiao-yan
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (1) : 61-66.
Study on the protective effect of Tongxinluo on kidneys of diabetic rats based on the expression of TGF- β1 and CTGF
Objective To invatigate the effect of Tongxinluo on transforming growth factor beta 1（TGF-β1）and connective tissue growth factor (CTGF) expression in diabetic rat kidney, and effect of tongxinluo on diabetic rats kidney protection. Methods The 40 healthy male SD rats were randomly divided into 2 groups, with 12 rats in the normal control group (CON group), and 28 rats in the model group. The model group was used to manufacture diabetes mellitus with a disposable tail vein injection of Streptozotocin (STZ) 55 mg/kg, and the blood glucose was greater than 16.7 mmol/L after 72 hours. 24 rats with successful modeling were randomly divided into diabetic group (DM group), and group (TXL) tongxinluo group, 12 rats in each group. 8 weeks later full automatic biochemical analyzer test was used for biochemical indexes and kidney tissue ultrastructure was observed using electron microscopice. Immunohistochemical method was to detect the expression of TGF-β1, and CTGF on protein level, Western blot method was used for determination of TGF-β1, and CTGF protein levels in kidney tissue. Results Compared with the diabetic group, the thickening of the renal basement membrane, the accumulation of mesangial matrix and the fusion of the foot were all reduced, and the expression of TGF-β1 and CTGF in renal tissues decreased. Conclusion Tongxinluo can reduce the expression of TGF-β1 and CTGF in kidney tissues of diabetic rats, and has protective effect on kidney of diabetic rats.
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