The expression and significance of active oxygen modulation factor 1 in human intervertebral disc degeneration
YAO Nu-zhao, TANG Shao-feng, XIA Xi, TAN De-rong
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (1) : 56-60.
The expression and significance of active oxygen modulation factor 1 in human intervertebral disc degeneration
Objective To explore the expression and their relationship of ROMO1 and ROS in the degenerative intervertebral disc. Methods Immunohistochemical techniques and fluorescent probes DCFH-DA were applied to detect the expression of ROMO1 and ROS levels in the different degenerated nucleus pulposus. Results The mean option density of ROMO1 were 0.192±0.089, 0.328±0.048, 0.399±0.053, and 0.468±0.098, respectively. The results of ROS detection were 132.961±15.149, 191.889±17.880, 218.056±12.845, and 243.501±30.279， separately，in the groups of A, B, C, and D. It showed the expression of ROMO1 and the content of ROS increased gradually with the aggravation of intervertebral disc degeneration, and there was a positive correlation between ROMO1 expression level and the content of ROS in intervertebral disc (r=0.732, P<0.05). There was also a positive correlation between ROMO1 expression level and the intervertebral disc degeneration degree (r=0.603, P<0.05). Conclusion ROMO1 and ROS are highly expressed in the nucleus pulposus tissue of the degenerated intervertebral disc, which has a positive correlation; and the expression level of ROMO1 is positively correlated with the degree of degeneration of intervertebral disc, which might be related to aggravated oxidative stress injury by increasing the content of ROS in the nucleus pulposus.
Reactive oxygen species modulator 1; Reactive oxygen species; Oxidative stress; / Intervertebral disc degeneration
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