The research of specified recognition of lectins with carbohydrate modified on surface markers of mouse embryonic stem cells
HU Ji-liang, WANG Hao, CHEN Jia, LV Wen, CHEN Dong
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (1) : 51-55.
The research of specified recognition of lectins with carbohydrate modified on surface markers of mouse embryonic stem cells
Objective Identification of lectins which can specified recognize the carbohydrate modified surface markers on mouse embryonic stem cells. Methods In the present study, a panel of 3 lectins and carbohydrate antibodies was used to characterize the carbohydrate surface markers of mouse Embryonic Stem (ES) Cells by Flow cytometry assay and Immunocytochemistry. Results Enrichment of mouse ES cells yielded approximately 99% of SSEA-1-positive mouse ES cells. A uniform and high percentage of binding was observed for DSL, having similar percentage of binding to SSEA-1 (99%). Partial binding of WFL was observed in mouse ES cells which was also reflected by the respective immunocytochemistry images. A very low percentage of binding was observed for UEAI. Conclusion DSL can be used to determine pluripotency which shows similar binding to SSEA-1, a well-established pluripotent marker and UEAI is a negative marker. Taken together, the data has provided information on the cell surface carbohydrate profile of mouse ES cells.
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