Objective　To explore the segmentation method of fetal hepatic vascular three-dimensional model and its significance.　Methods　One case of fresh induced labor normal fetal liver（38 week）was infused with color separation and density contrast metal contrast agent and then casted, red epoxy resin filler perfusion of abdominal aorta (contrast agent for lead oxide), blue epoxy resin filler perfusion of inferior vena cava, ductus venosus, left hepatic vein and lateral branch of right hepatic vein (contrast agent for titanium dioxide), purple epoxy resin filler perfusion of intermediate hepatic vein(contrast agent for zinc oxide), green epoxy resin filler perfusion of interior branch of right hepatic vein(contrast agent for lead oxide).the 128-slice spiral CT thin layer scanning and Mimics software were used to reconstruct three dimensional model of fetal liver.　Results　The three dimensional model of fetal hepatic vessels was constructed, which were clearly and solid. When the Mimics threshold spacing was progressively smaller, it can effectively segment the intermediate hepatic vein, the hepatic vein system and the hepatic proper artery, which can clearly show the course and distribution of the multiple vessels in fetal liver.　Conclusion　 It is simple and feasible to apply different density contrast agents to perfusion of multiple vessels in fetal liver, which can provide ideal technical support for the study of the development and transplantation of fetal hepatic vessels.