Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection
MIAO Ying-ying, LU Ming, ZHAO Jun-qiang
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (6) : 645-649.
Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection
Objective Erythropoietin (EPO) has been reported to have a beneficial effect on obesity. The aim of the study is to investigate the changes of body weight and fat content of EPO in high fat diet (HFD) mice, the expression of PPAR γ, UCP1, β3-AR mRNA in white adipose tissue (WAT) and brown adipose tissue (BAT). Methods 20 HFD-fed C57BL/6J male mice were randomly divided into control group (HFD-Con) and EPO group (HFD-EPO). The expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous fat, epididymal fat and brown fat were detected by real-time quantitative RT-PCR. Results Compared with the control group, the body weight of the EPO mice was (24.53±2.03) g and the control group was (31.78±1.68) g. The weight of subcutaneous fat and epididymal fat in HFD-EPO group were significantly decreased. HE staining showed that the size of adipocytes of HFD-EPO group was decreased compared with HFD-Con group. Quantitative real-time PCR results showed that the expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous adipose tissue and epididymal adipose tissue did not change significantly, while the expression of PPAR γ, UCP1 and β3-AR in brown fat were significantly increased. Conclusion The expression of PPAR γ, UCP1 and β3-AR in brown fat may be related to the weight loss caused by EPO injection after high fat diet.
High fat diet / Erythropoietin / Peroxisome proliferator-activated receptors &gamma / Uncoupling protein 1 / &beta / 3-adrenergic receptor
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