The mechanical effect and mechanism of leonurine on rat mesenteric artery
XU Yun-fei, WU Yun, LI Wen-chen
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (5) : 548-553.
The mechanical effect and mechanism of leonurine on rat mesenteric artery
Objective To investigate the mechanical effect and mechanism of leonurine on rat mesenteric artery. Methods 50 healthy male Sprague-Dawley rats were randomly divided into the control group, the low, middle and high dose groups, with 10 rats in each group, and the other 10 in vitro experiments. Leonurine group were given leonurine saline suspension, and the control group was given normal saline. Four weeks later, enzyme linked immunosorbent assay was adopted for analysis of the content of nitric oxide in the serum and endothelin-1 in the plasma. The 3rd mesenteric artery was harvested and stained by hematoxylin-eosin. Multi Wire Myograph System was used to observe the relaxation effect of leonurine on endothelium-intact and endothelium-denuded third rats mesenteric artery rings, and the relaxation effect with inhibitor of nitric oxide synthase (L-NAME), soluble guanylate cyclase (sGC) inhibitor (NS-2028) and calcium channel antagonist nifedipine incubated. Results Compared with the control group, the content of nitric oxide (NO) in serum and the content of endothelin -1 (ET-1) in plasma of leonurine group decreased. As the dose of leonurine increases, the ET-1 decreased faster. Hematoxylin-eosinstaining showed the blood vessel wall of the Leonurine group was thinner than that of the saline control group, and the effect is dose-dependent. Leonurine can directly relax the endothelium-intact isolated mesenteric artery rings. The relaxation effect of leonurine on the endothelium-denuded isolated mesenteric artery was obvious and with incubation of inhibitor of nitric oxide synthase (L-NAME), soluble guanylate cyclase (sGC) inhibitor (NS-2028) and calcium channel antagonist nifedipine, the relaxation effect was significantly reduced. Conclusion Leonurineintragastric administration has a protective effect on rat mesenteric artery. The vasodilation effect of leonurine on isolated mesenteric artery rings was concentration dependent and endothelium dependent. This effect may be related to NO-sGC-cGMP signaling pathway and L-type calcium channel.
Leonurine / Mesenteric artery / Nitric oxide / Multi Wire Myograph System
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