Experimental study on the application of mineralized collagen and magnesium calcium alloy composite in the preservation of dog tooth extraction site
YU Qing, WANG Cheng-yue, YANG Jing-xin, GUO Chang-wu
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (5) : 532-536.
Experimental study on the application of mineralized collagen and magnesium calcium alloy composite in the preservation of dog tooth extraction site
Objective To study the feasibility of mineralized collagen composite calcium magnesium alloy used in the preservation of dog tooth extraction site. Methods Forth premolars of eight mongrel dogs from the left and right sides of the mandible were extracted, which then resulted in a total of 32 extraction sockets that were then randomly divided into two groups, A and B, with 16 extraction sockets in each group. Group A was implanted with the composite material of mineralized collagen and calcium magnesium alloy, group B was only implanted with mineralized collagen material, the dog was sacrificed 12, 24 weeks later to prepare tissue specimens. The healing of sockets was evaluated by gross observation,CBCT and X-ray microscopes. The damage of liver and kidney tissues was observed by histological sections of liver and kidney. Results After implantation of the two groups of materials in the extraction nest, with the healing of the tooth socket, the material gradually degraded and absorbed, the new bone tissue continued to form. At 12 weeks postoperatively, the relative bone mineral density and the number of new bone tissues in experimental group A were significantly higher than those in control group B (P<0.05). At 24 weeks after operation, the number of new bone tissue in group A and B increased gradually, and the difference of bone mineral density between the two groups was not significant (P>0.05). Conclusion The composite material of mineralized collagen and calcium magnesium alloy can be used for preservation of dog 's tooth extraction site, which promotes the regeneration of new bone and reduces the absorption of alveolar ridge.
Mineralized collagen / Calcium magnesium alloy / Tooth-extraction site / Bone regeneration
[1] 张晓倩, 尼加提·吐尔逊, 王艳, 等. 骨诱导活性材料用于牙槽嵴保存的动物实验研究[J]. 医学研究杂志, 2014, 4(11):63-66.
[2] Li J, Hong J, Zheng Q, et al. Repair of rat cranial bone defects with nHAC/PLLA and BMP-2-related peptide or rhBMP-2[J]. J Orthop Res, 2011, 29(11):1745-1752.
[3] Sun Y, Wang CY, Wang ZY, et al. Test in canine extraction site preservations by using mineralized collagen plug with or without membrane [J].J Biomater Appl, 2016,30(9):1285-1289.
[4] 王程越，赵远，杨曼，等. 纳米晶胶原人工骨用于新疆维吾尔自治区塔城地区哈萨克族人的牙槽嵴保存[J].中国组织工程研究，2015，19(12):1864-1871.
[5] 王韦丹，韩军杰，万鹏，等. 可降解镁合金作为骨填充材料的研究和展望[J].中国医疗设备, 2015, 30(9):12-15.
[6] Ioku Y, Haeniwa H, Kakudo K, et al. Effect of β-tricalcium phosphate and porous hydroxyapatite bone substitutes on bone regeneration in alveolar bone defects around dental implants[J]. J Oral Maxillofac Surg, 2015, 49(9):69-84.
[7] 孙翼，王程越，王彦夫，等. 新型复合矿化胶原膜用于牙槽嵴保存的实验研究[J].实用口腔医学杂志, 2016, 32(5):645-649.
[8] Han X, Liu H, Wang D, et al. Alveolar bone regeneration around immediate implants using an injectable nHAC/CSH loaded with autogenic blood-acquired mesenchymal progenitor cells:An experimental study in the dog mandible[J]. Clin Implant Dent Relat Res, 2013, 15(3):390-401.
[9] Hennessy KM, Pollot BE, Clem WC, et al. The effect of collagen I mimetic peptides on mesenchymal stem cell adhesion and differentiation, and on bone formation at hydroxyapatite surfaces[J]. Biomaterials, 2013, 30(10): 1898-1909.
[10] Staiger MP，Pietak AM，Huadmai J，et al. Magnesium and its alloys as orthopedic biomaterials：a review[J]. Biomaterials，2006，27(9)：1728-1734.
[11] Marie PJ. The calcium-sensing receptor in bone cells: A potential therapeutic target in osteoporosis[J]. Bone, 2010, 46(3): 571-576.
[12] He G, Wu Y, Zhang Y, et al. Addition of Zn to the ternary Mg-Ca-Sr alloys significantly improves their antibacterial property[J]. J Mater Chem B Mater Biol Med, 2015, 3(32): 6676–6689.
[13] Qiu ZY, Cui Y, Tao CS, et al. Mineralized Collagen: Rationale, Current Status, and Clinical Applications[J]. Materials, 2015, 8(8):4733-4750.
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