Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI
TIAN Zhi-hui, LIAO Wei-guo, YU Jian-ye, WANG Li-sheng
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (4) : 409-412.
Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI
Objective To investigate the effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow. Methods Laser speckle imaging system (LSCI) was used to detect the effects of different ischemic duration on post-ischemia and reperfusion rat cerebral blood flow. At the same time, the dynamic changes of capillary flow and diameter, no reflow phenomenon and hypoperfusion were analyzed. Results During 2h middle cerebral artery occlusion (MCAO) ischemia, perfusion of cerebral blood flow decreased to (35±10)% of the baseline, reflecting a state of hypoperfusion. With the prolongation of ischemic time, no reflow phenomenon was aggravated. Hypoperfusion in 2 h group was approximately 10% less than that in 30 min group during the period of ischemia and reperfusion. Conclusion The long ischemic duration on post-ischemic and reperfusion may be one of the main reasons for the increase of the degree of brain injury.The established LSCI method can be applied to real-time monitoring of MCAO rat cerebral blood flow, which is easy to operate, sensitive, stable and reliable.
Cerebral ischemia-reperfusion / Laser speckle imaging / Cerebral blood flow
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