The protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by amphetamine
CHI Dan-lu1， SUN Yu1， PAN San-qiang2
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (4) : 397-401.
The protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by amphetamine
Objective To investigate the protective effect and its mechanism of Antrodia camphorata enzyme on the cytotoxicity injury of PC12 cells induced by Amphetamine (AMPH). Methods PC12 cells were treated with 3.5 mmol/L AMPH or 3.5 mmol/L AMPH pus Antrodia camphorata enzyme. The morphological changes of the cells were observed under the light microscope. Cell viability assay was measured by MTT method. Caspase-3 levels were detected by Western blot, and the changes of reactive oxygen species（ROS） and apoptosis of cells were analyzed by flow cytometer. Results (1)AMPH damaged structure of PC12 cells, and resulted in cell apoptosis. Antrodia camphorata enzyme reduced cell apoptosis, and increased the survival rate of cells. (2)Antrodia camphorata enzyme reduced generation of ROS in PC12 cells. (3)Compared to AMPH group, procaspase-3 (35 kd) level was increased while procaspase-3 (17 kd) level was reduced in Antrodia camphorata enzyme group. Conclusion Antrodia camphorata enzyme has a protective effect on the cytotoxicity of PC12 cells induced by AMPH. Its mechanism may be related to inhibition of the mitochondrial apoptosis signal pathway and reduction of reactive oxygen species in cells.
Antrodia camphorata enzymes / Amphetamine; / PC12 cell; / Apoptosis; / Reactive oxygen species
[1] UNODC. World drug report 2016 [M]. New York: United Nations publication,2016: 52.
[2] Lu MC, El-Shazly M, Wu TY, et al. Recent research and development of Antrodia cinnamomea [J]. Pharmacol Ther, 2013,139(2):124-156.
[3] Wang LC, Wang SE, Wang JJ, et al. In vitro and in vivo comparisons of the effects of the fruiting body and mycelium of Antrodia camphorata against amyloid β-protein-induced neurotoxicity and memory impairment [J]. Appl Microbiol Biotechnol, 2012, 94(6):1505-1519.
[4] McCord JM, Edeas MA. SOD, oxidative stress and human pathologies: a brief history and a future vision [J]. Biomed Pharmacother, 2005, 59(4):139-142.
[5] Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes [J]. Curr Med Chem，2000, 7(7):715-729.
[6] Geethangili M, Tzeng YM. Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds[J]. Evid Based Complement Alternat Med, 2011, 212641.
[7] Ao ZH, Xu ZH, Lu ZM, et al. Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases [J]. J Ethnopharmacol, 2009,121(2):194-212.
[8] 任亚丽，宿宝贵，马丽华，等. 苯丙胺致小鼠行为学的变化和纹状体纤维的损伤[J]. 中国临床解剖学杂志，2012, 30 (3):320-323.、
[9] Cunha-Oliveira T, Rego AC, Oliveira CR. Cellular and molecular mechanisms involved in the neurotoxicity of opioid and psychostimulant drugs [J]. Brain Res Rev, 2008, 58(1):192-208.
[10] Krasnova IN, Ladenheim B, Cadet JL. Amphetamine induces apoptosis of medium spiny striatal projection neurons via the mitochondria-dependent pathway [J]. FASEB J, 2005, 19(7):851-853.
[11] 江海峰，赵敏，孙海明，等. 苯丙胺类兴奋剂滥用的治疗研究进展[J].中国药物依赖性杂志, 2008, 17 (4) : 259-262.
[12] 高磊，周文华. 苯丙胺类兴奋剂依赖的临床治疗的进展 [J]. 中国药物依赖性杂志, 2016, 25(6):491-496.
[13] Shalini S, Dorstyn L, Dawar S, et al. Old, new and emerging functions of caspases [J]. Cell Death Differ, 2015, 22(4):526-539.
[14] Sánchez-Migallón MC, Valiente-Soriano FJ, Nadal-Nicolás FM, et al. Apoptotic retinal ganglion cell death after optic nerve transection or crush in mice: delayed RGC loss with BDNF or a caspase 3 Inhibitor [J]. Invest Ophthalmol Vis Sci, 2016,57(1):81-93.
[15]Carvalho M, Carmo H, Costa VM, et al. Toxicity of amphetamines: an update[J]. Arch Toxicol, 2012, 86(8):1167-1231.
[16]Liu Y, Wang J, Li L, et al. Hepatoprotective effects of Antrodia cinnamomea: The modulation of oxidative stress signaling in a mouse model of alcohol-induced acute liver injury [J]. Oxid Med Cell Longev, 2017,7841823.
/
| 〈 |
|
〉 |
