Expression of Beclin1 and LC3 in dexamethasone-induced rat critical illness myopathy
QU Hui-ying, BAO Cui-fen, YU Di, QIN Shu-jian
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3) : 276-281.
Expression of Beclin1 and LC3 in dexamethasone-induced rat critical illness myopathy
Objective To investigate the expression of critical illness myopathy on autophagy-related factors Beclin1 and LC3 in rat skeletal muscle. Method SD rats were divided into control and experimental groups; Experimental groups were divided into 3 time points, i.e., 7, 9 and 11d（n=10）.Experimental groups underwent intraperitoneal injection of dexamethasone 5 mg/kg once per day, and the healthy control group was injected with normal saline. The muscle function defect was determined by footprint method. The expression of skeletal muscle autophagy-related factors Beclin1 and LC3 was examined by immunohistochemistry and western blot analysis. Results Compared with the control group, rats in the experimental groupdemonstrated muscle function impairment symptoms of varying degrees ,which was most serious at 11d time point. Western blot results showed that Beclin1 and LC3 positive expression in the control group, or Beclin1 and LC3 expression were significantly higher in the experimental group than that in the control group. Immunohistochemistry confirmed the same trend. Conclusion Beclin1 and LC3 may play an important role in cell autophagy of skeletal muscle in critical illness myopathy in rats.
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