The bio-reactivity analysis of HSNGLPL peptide grafted-polyurethane
XIAO Jiang-wei, SHI Dan-dan, ZHU Rong, GU Rui-cai, WU Gang, LIAO Hua
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3) : 271-275.
The bio-reactivity analysis of HSNGLPL peptide grafted-polyurethane
Objective To study the biocompatibility of HSNGLPL peptide grafted- polyurethane in vivo and in vitro. Methods HSNGLPL peptide solution, HSNGLPL peptide grafted polyurethane with different concentration, and BDO- PU are injected or implanted into tibialis anterior muscle of B6 mice, or co-cultured with C2C12 cell in vitro. CCK8, H&E, immunofleorecence was used to explore the material-induced inflammatory and immuno-reactivity in vitro and in vivo. Results HSNGLPL peptide grafted-polyurethane is biocompatible in vivo. BDO-PU induced a conspicuous monocytes/macrophages infiltration and myofiber degeneration, the inflammatory invasion and myofiber necrosis are mainly detected in the site around the implants. In contrast, peptide grafted-PUs induced intramuscular inflammation is more complex and sustained more than 56d. The number of apoptotic macrophages in muscle containing peptide-PU significantly lower than that containing BDO-PU. Conclusions HSNGLPL peptide grafted PUs triggers the more severe inflammation response than that of BDO-PU, and eventually change biocompatibility of PU material in vitro and in vivo.
Polyurethanes / HSNGLPL peptide; Inflammation; / Immunity; / Infiltration
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