维生素C对巨噬细胞增殖、迁移及吞噬功能的影响

doi:10.13418/j.issn.1001-165x.2017.03.007

Abstract

Abstract:

Objective　To investigate the effects of Vitamin C (VC) on macrophage proliferation, migration and phagocytic activity.　Methods　Macrophages isolated from SD rats were cultured in a basic medium supplement with or without VC for 24 hours. Then the proliferation of macrophages was assessed by BrdU and WST assays, while the phagocytic activity was detected by the phagocytosis of lumispheres which were administrated into the culture medium for 3 hours, and the migration was measured by scratching test and Transwell assay.　Results　The percentage of BrdU positive cells was significantly increased in the VC treated group while the intensity of WST test was higher in VC group than that of control group (P<0.05). The number of lumispheres in each macrophage of VC group was much more than that of control group(P<0.05). Moreover, VC could accelerate the migrating rate of macrophages when the cells were tested by scratching test and Transwell assay (P<0.05).　Conclusion　VC could enhance the proliferation, migration and phagocytosis of macrophages.

Key words: Vitamin C, 　Macrophage, 　Proliferation, 　Migration, 　Phagocytic activity

FAN Zhi-hao, LI Yuan-yuan, LI Li-xia,WANG Xiang-hai, PAN Meng-jie, TAN Dan-dan, WEN Jin-kun, QIAN Chang-hui, LI Zhen-lin，GUO Jia-song. The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(3): 266-270.

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The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity

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