The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity
FAN Zhi-hao, LI Yuan-yuan, LI Li-xia,WANG Xiang-hai, PAN Meng-jie, TAN Dan-dan, WEN Jin-kun, QIAN Chang-hui, LI Zhen-lin，GUO Jia-song
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3) : 266-270.
The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity
Objective To investigate the effects of Vitamin C (VC) on macrophage proliferation, migration and phagocytic activity. Methods Macrophages isolated from SD rats were cultured in a basic medium supplement with or without VC for 24 hours. Then the proliferation of macrophages was assessed by BrdU and WST assays, while the phagocytic activity was detected by the phagocytosis of lumispheres which were administrated into the culture medium for 3 hours, and the migration was measured by scratching test and Transwell assay. Results The percentage of BrdU positive cells was significantly increased in the VC treated group while the intensity of WST test was higher in VC group than that of control group (P<0.05). The number of lumispheres in each macrophage of VC group was much more than that of control group(P<0.05). Moreover, VC could accelerate the migrating rate of macrophages when the cells were tested by scratching test and Transwell assay (P<0.05). Conclusion VC could enhance the proliferation, migration and phagocytosis of macrophages.
Vitamin C / Macrophage / Proliferation / Migration / Phagocytic activity
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