Effects of herbal steam-wash therapy on IL-1β and TGF-β1 concentrations in the knee joint fluids of knee osteoarthritis

WANG Guan-jie, ZHANG Lei, XIE Li-jun, ZHOU Xin, LIU Gang, GUO Xiao-guang, FU Shi-jie

Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 193-198.

Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 193-198. DOI: 10.13418/j.issn.1001-165x.2017.02.015

Effects of herbal steam-wash therapy on IL-1β and TGF-β1 concentrations in the knee joint fluids of knee osteoarthritis

  • WANG Guan-jie 1,2,  ZHANG Lei1, XIE Li-jun1,  ZHOU Xin1, LIU Gang1, GUO Xiao-guang1, FU Shi-jie1
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Abstract

Objectives To observe the effects of herbal steam-wash therapy on knee osteoarthritis(KOA),in terms of WBC, IL-1β and TGF-β1 concentrations. Methods 9 SPF old cynomolgus monkeys as KOA animal models were randomly divided into a steam-wash group (n=3), a traditional group(n=3) and a control group(n=3). Other 6 SPF youth cynomolgus monkeys were selected as a healthy group. The left knee of cynomolgus monkeys in the steam-wash group or traditional group were treated with herbal steam-wash therapy or traditional herbal compress therapy for 6 weeks, separately. The WBC, IL-1β and TGF-β1 concentration were detected from joint irrigation fluids before treatment and 2 weeks, 4 weeks, 6 weeks after treatment. Analysis of variance for repeated data was performed. Results (1)Articular cartilage degeneration of aged cynomolgus monkeys could be seen by HE staining; (2) In regard to WBC and IL-1β concentration before treatment, the steam-wash group, traditional group and control group compared with the normal group was significantly decreased (P<0.05). On the contrast, the concentration of TGF-β1 increased significantly (P<0.05). (3) At 2 weeks, 4 weeks, 6 weeks after treatments, WBC and IL-1β concentration decreased significantly than those before treatments in the steam-wash group and traditional group (P<0.05). (4) In regard to WBC and IL-1β concentration at 2 weeks, 4 weeks, 6 weeks after treatments, the steam-wash group and traditional group compared with the control group were significantly decreased (P<0.05), which decreased more in the steam-wash group compared with the traditional group (P<0.05). (5) The TGF-β1 concentration increased significantly at 2 weeks and 4 weeks after treatments (P<0.05). (6) In terms of TGF-β1 concentration at 2 weeks, 4 weeks after treatments, the steam-wash group and traditional group compared with the control group were significantly increased (P<0.05), which increased more in the steam-wash group compared with the traditional group (P<0.05).    Conclusions The IL-1β, TGF-β1 concentrations and WBC in the knee joint fluids from cynomolgus monkey models of KOA were abnormal. Herbal steam-wash therapy and traditional herbal compress therapy could decrease the IL-1β concentration and increase the TGF-β1 concentration in knee joint, which is helpful for treatment of KOA. Moreover, the effect of herbal steam-wash therapy is better than traditional herbal compress therapy.

Key words

KOA / Steam-wash therapy / WBC / IL-1β / TGF-β1

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WANG Guan-jie, ZHANG Lei, XIE Li-jun, ZHOU Xin, LIU Gang, GUO Xiao-guang, FU Shi-jie. Effects of herbal steam-wash therapy on IL-1β and TGF-β1 concentrations in the knee joint fluids of knee osteoarthritis[J]. Chinese Journal of Clinical Anatomy. 2017, 35(2): 193-198 https://doi.org/10.13418/j.issn.1001-165x.2017.02.015

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