Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine
ZHANG Yi-min, LIAN Hui, FU Sheng-qi, REN Tong-ming, CAI Jun-yan, WANG Zhi-yong
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 188-192.
Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine
Objective To observe the expression of ionized calcium binding adapter molecule-1 (Iba1) in rat paraventricular nucleus of hypothalamus (PVN) induced by substantia nigra lesion by 6-hydroxydopamine (6-OHDA). Methods According to random number table, a total of 30 male SD rats were randomly divided into a 6-OHDA group and a normal control group. 6-OHDA was injected into the bilateral substantia nigra (SN) of the rats in the 6-OHDA group, and the same volume of saline was injected into the same position of the rats in the normal control group. After sixweeks, the rats were sacrificed and the brains were obtained. Nissl staining was used to detect the Nissl bodies in the SN, and immunofluorescent assay and Western blot analysis were used to detect TH expression in the SN and Iba1 expression in the PVN, and RT-PCR were used to detect Iba1 in the PVN. Results Compared with the normal control group, the number of TH immunoreactive neurons in the SN of the 6-OHDA group decreased from (58±5) to (10±2) (P<0.05), and the protein level of TH decreased from (0.71±0.1) to (0.28±0.12) (P<0.05). The number of Iba1 immunoreactive cells in the PVN of the 6-OHDA group increased from (7±2) to (14±3) (P<0.05), and the level of Iba1 protein increased from (0.1±0.08) to (0.19±0.06) (P<0.05). Conclusion The changes of Iba1 expression in PVN may be associated with gastric emptying dysfunction in patients with Parkinson’s disease.
Parkinson's disease / Paraventricular nucleus of hypothalamus / Ionized calcium binding adapter molecule-1
[1] Pfeiffer RF. Non-motor symptoms in Parkinson's disease [J]. Parkinsonism Relat Disord, 2016, 22,1:S119-122.
[2] Garden GA, Möller T. Microglia biology in health and disease[J]. J Neuroimmune Pharmacol, 2006, 1(2):127-137
[3] Iida H, Doiguchi M, Yamashita H, et al. Spermatid-specific expression of Iba1, an ionized calcium binding adapter molecule-1, in rat testis[J]. Biol Reprod, 2001, 64(4):1138-1146.
[4] Ichitani Y, Okamura H, Nakahara D. Biochemical and immunocytochemical change induced by intrastriatal 6-hydroxydopamine injection in the rat nigrostriatal dopamine neuron system: evidence for cell death in the substantia nigra [J]. J Exp Neurol, 1994, 130(2):269-279
[5] Valkovic P, Minar M, Singliarova H, et al. Pain in Parkinson's Disease: A Cross-Sectional Study of Its Prevalence, Types, and Relationship to Depression and Quality of Life [J]. PLoS One, 2015, 10(8):1-11.
[6] Xu Z, Wang BR, Wang X, et al. ERK1/2 and p38 mitogen-activated protein kinase mediate iNOS-induced spinal neuron degeneration after acute traumatic spinal cord injury [J]. Life Sci, 2006,79 (20):1895-1905.
[7] Hay, N Sonenberg, N. Upstream and downstream of mTOR [J]. Genes Dev, 2004, 18(16): 1926-1945.
[8] Xu B, Guan XH, Yu JX, et al. Activation of spinal phosphatidylinositol 3-kinase/protein kinase B mediates pain behavior induced by plantar incision in mice [J]. Exp Neurol, 2014, 255: 71-82.
[9] Pellegrini C, Antonioli L, Colucci R, et al. Gastric motor dysfunctions in Parkinson's disease: Current pre-clinical evidence[J]. Parkinsonism Relat Disord, 2015, 21(12):1407-1414.
[10] Fasano A, Visanji NP, Liu LW, et al. Gastrointestinal dysfunction in Parkinson's disease[J]. Lancet Neurol, 2015, 14(6): 625-639.
[11] Hao H, Luan X, Guo F, et al. Lateral hypothalamic area orexin-A influence the firing activity of gastric distension-sensitive neurons and gastric motility in rats[J]. Neuropeptides, 2016, 57:45-52
[12] Guo FF, Xu L, Gao SL, et al. The effects of nesfatin-1 in the paraventricular nucleus on gastric motility and its potential regulation by the lateral hypothalamic area in rats[J]. J Neurochem, 2015, 132(3):266-275
[13] Guo F, Xu L, Sun X, et al. The paraventricular nucleus modulates thyroidal motilin release and rat gastric motility [J]. J Neuroendocrinol, 2011, 23(9):767-777.
[14] Thaler JP, Yi CX, Schur EA, et al. Obesity is associated with hypothalamic injury in rodents and humans[J]. J Clin Invest, 2012, 122(1):153-162.
[15] Delgado R, Carlin A, Airaghi L, et al. Melanocortin peptides inhibit production of proinflammatory cytokines and nitric oxide by activated microglia [J]. J Leukoc Biol, 1998, 63(6):740-745.
[16] Ferreira R, Santos T, Viegas M, et al. Neuropeptide-Y inhibits interleukin-1beta-induced phagocytosis by microglial cells [J]. J Neuroinflammation, 2011, 8: 169.
[17]Gonçalves J, Ribeiro CF, Malva JO, et al. Protective role of neuropeptideY Y2 receptors in cell death and microglial response following methamphetamine injury [J]. Eur J Neurosci, 2012, 36(9):3173-3183.
/
| 〈 |
|
〉 |
