Establishment and identification of myeloid cell-specific SETD4 knockout mice
HUANG Meng-yi, ZHONG Yu-yun, HUANG Sui, Sun Jiang, Li Yue, WANG Juan, JIANG Yong, LIU Jing-hua
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (2) : 177-182.
Establishment and identification of myeloid cell-specific SETD4 knockout mice
Objective To elucidate the function of SETD4, we established myeloid cell-specific SETD4 knockout mice. Methods Setd4flox/+ mice were inbred to obtain Setd4flox/flox mice, which then bred with FLP mice to obtain the Setd4fl/+/flp mice. After crossing with C57BL/6 mice, Setd4fl/+ mice were obtained, while Setd4fl/+/Lyz2-Cre were obtained after crossing with Lyz2-Cre mice. And then Setd4-/-/Lyz2-Cre mice were generated by mating Setd4fl/+/Lyz2-Cre with Setd4fl/+ mice. PCR was used to identify the genotype of offspring. The mRNA level of SETD4 in peritoneal macrophages and liver were detected by quantitative real time PCR to confirm the knockout efficiency. Result The mRNA expression of SETD4 in peritoneal macrophages of myeloid cell-specific SETD4 knockout mice was significantly lower than the wildtype mice, while no significant difference could be detected in the liver. Conclusion Based on FLP/FRT、Cre/Loxp recombination system, myeloid cell-specific SETD4 knockout mice were successfully established for further research.
FLP/FRT / Cre/loxp / SETD4 / Gene knockout
[1] Kouzarides T. Histone methylation in transcriptional control[J]. Curr Opin Genet Dev, 2002,12(2): 198-209.
[2] Xiao B, Wilson JR, Gamblin SJ. SET domains and histone methylation[J]. Curr Opin Struct Biol, 2003,13(6): 699-705.
[3] Jenuwein T, Laible G, Dorn R, et al. SET domain proteins modulate chromatin domains in eu-and heterochromation[J]. Cell Mol Life Sci,1998,54(1): 80-93.
[4] Xu G, Liu G, Xiong S, et al. The histone methyltransferase Smyd2 is a negative regulator of macrophage activation by suppressing interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) production[J]. J Biol Chem, 2015, 290(9): 5414-5423.
[5] Wang X, Zhu K, Li S, et al. MLL1,a H3K4 methyltransferase regulates the TNFα-stimulated activation of genes downstream of NF-κB[J]. J Cell Sci, 2012, 125(17): 4058-4066.
[6] Levy D, Kuo AJ, Chang Y, et al. Lysine methylation of the NF-κB subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-κB signaling[J]. Nat Immunol, 2011,12(1): 29-36.
[7] Bayarsaihan D. Epigenetic mechanisms in inflammation[J]. J Dent Res, 2011, 90(1): 9-17.
[8] Hachiya R, Shiihashi T, Shirakawa I, et al. The H3K9 methyltransferase Setdb1 regulates TLR4-mediated inflammatory responses in macrophages[J]. Sci Rep, 2016, 6: 28845.
[9] Xia M, Liu J, Wu X, et al. Histone methyltransferase Ash1L suppresses interleukin-6 production and inflammatory autoimmune diseases by inducing the ubiquitin-editing enzyme A20[J]. Immunity, 2013, 39(3): 470-481.
[10] Hamamoto R, Furukawa Y, Morita M, et al. SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells[J]. Nat Cell Biol, 2004, 6(8): 731-740.
[11] Faria JA, Corrêa NC, de Andrade C, et al. SET domain-containing protein 4 (SETD4) is a newly identified cytosolic and nuclear lysine methyltransferase involved in breast cancer cell proliferation[J]. J Cancer Sci Ther, 2014, 5(2): 58-65.
[12] 叶萍, 蔡军伟, 付晓霞, 等. p38信号通路对SETD4在细胞中的表达和定位的影响[J]. 中国病理生理杂志, 2012, 28(5): 878-883.
[13] 雷烨铭, 崔航, 钟玙沄, 等. SETD4蛋白在Bac-to-Bac杆状病毒系统的表达[J]. 中国临床解剖学杂志, 2015, 33(4): 426-429.
[14] 黄穗, 黄梦怡, 钟玙沄, 等. SETD4基因敲除小鼠的构建与鉴定[J]. 中国临床解剖学杂志, 2016, 34(2): 202-207.
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